F11 ELISA Kit (Rat) (GWB-KBBFU7)

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96 Wells

Additional Information

Name F11 ELISA Kit (Rat) (GWB-KBBFU7)
Kit Range 0.156-10 ng/mL
Sensitivity <0.067 ng/mL
Sample Type Serum, plasma
Kit Detection Absorbance, OD 450 nm
Gene Symbol F11
Gene id 290757
Alias Symbols LRRGT00086
Swissprot ID Q6TUF8
Protein Name coagulation factor XI
Kit Principle Genway Biotech F11 ELISA Kit (Rat) (GWB-KBBFU7) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for F11 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for F11 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample F11 captured in the well.
Kit Component
Anti-F11 Microplate96 Wells (12 x 8 Well Strips)
F11 Lyophilized Standard2 x 10 ng
Standard Diluent1 x 20 mL
100X F11 Biotinylated Detector Antibody1 x 120 uL
100X Avidin-HRP Detection Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Detection Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001041313.1
Nucleotide Accession Num NM_001047848.1
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3.5 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBFU7
Species Reactivity Rat