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96 Wells

Additional Information

Name FBXL3 ELISA Kit (Mouse) (GWB-KBBUB6)
Kit Range 0.156-10 ng/mL
Sensitivity <0.059 ng/mL
Sample Type Serum, plasma, tissue homogenates
Kit Detection Absorbance, OD 450 nm
Gene Symbol FBXL3
Gene id 50789
Alias Symbols Afh, AU041772, AW212966, FBK, Fbl3a, F-box/LRR-repeat protein 3, F-box/LRR-repeat protein 3A, F-box and leucine-rich repeat protein 3A, Fbxl3a, Ovtm, Protein after-hours, Protein overtime
Swissprot ID Q8C4V4
Protein Name F-box/LRR-repeat protein 3
Description of Target Substrate-recognition component of the scf(fbxl3) e3 ubiquitin ligase complex involved in circadian rhythm function. plays a key role in the maintenance of both the speed and the robustness of the circadian clock oscillation. the scf(fbxl3) complex mainly
Kit Principle Genway Biotech FBXL3 ELISA Kit (Mouse) (GWB-KBBUB6) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for FBXL3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for FBXL3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample FBXL3 captured in the well.
Kit Component
Anti-FBXL3 Microplate96 Wells (12 x 8 Well Strips)
FBXL3 Lyophilized Standard2 x 10 ng
Standard Diluent1 x 20 mL
100X FBXL3 Biotinylated Detector Antibody1 x 120 uL
100X Avidin-HRP Detection Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Detection Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_056637.1
Nucleotide Accession Num NM_015822.2
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3.5 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBUB6
Species Reactivity Mouse