FDPS ELISA Kit (Human) (GWB-KBBWA3)

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GWB-KBBWA3

96 Wells
$659.00
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Additional Information

Name FDPS ELISA Kit (Human) (GWB-KBBWA3)
Kit Range 62.5 - 4,000 pg/mL
Sensitivity < 28.3 pg/mL
Sample Type serum, plasma, tissue homogenates and other biological fluids.
Kit Detection Colorimetric, OD 450 nm
Gene Symbol FDPS
Gene id 2224
Alias Symbols (2E,6E)-farnesyl diphosphate synthase, Dimethylallyltranstransferase, Farnesyl diphosphate synthase, Farnesyl pyrophosphate synthase, FPPS, FPP synthase, FPS, Geranyltranstransferase, KIAA1293
Swissprot ID P14324
Protein Name farnesyl pyrophosphate synthase
Description of Target Key enzyme in isoprenoid biosynthesis which catalyzes the formation of farnesyl diphosphate (FPP), a precursor for several classes of essential metabolites including sterols, dolichols, carotenoids, and ubiquinones. FPP also serves as substrate for protei
Kit Principle Genway Biotech FDPS ELISA Kit (Human) (GWB-KBBWA3) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for FDPS has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for FDPS is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample FDPS captured in the well.
Kit Component
ComponentAmount
Anti-RETNLB Microplate96 Wells (12 x 8 Well Strips)
RETNLB Lyophilized Standard2 x 4 ng
100X Biotinylated RETNLB Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001129293
Nucleotide Accession Num NM_001135821
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Kit linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)96-104%94-101%83-102%83-90%
EDTA Plasma (n=5)87-98%97-105%81-97%83-95%
Heparin Plasma (n=5)81-97%97-104%78-89%85-93%
Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)98-105102
EDTA Plasma (n=5)80-9489
Heparin Plasma (n=5)80-10196
Datasheets/Manuals Printable datasheet for GWB-KBBWA3
Species Reactivity Human