Feline Haptoglobin ELISA (GWB-374Z09) (Aviva Cat. No. OKIA00012)

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Please see item number OKIA00012 to view product details and for ordering information.


GWB-374Z09

Aviva Cat. No.: OKIA00012

Additional Information

Name Feline Haptoglobin ELISA (GWB-374Z09)
Related Product Names Haptoglobin alpha chain. Haptoglobin beta chain. HP2-ALPHA, HP, Cat Haptoglobin ELISA kit
Kit Range 6.25 ng/ml - 400 ng/ml
Sensitivity 4.261 ng/ml
Sample Type Serum, plasma and other biological fluids
Gene id 100144610
Alias Symbols Haptoglobin, HP, Zonulin
Swissprot ID E3UTY9
Protein Name Haptoglobin
Description of Target As a result of hemolysis, hemoglobin is found to accumulate in the kidney and is secreted in the urine. Haptoglobin captures, and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage. Haptoglobin also a
Kit Principle Genway Biotech Haptoglobin ELISA Kit (Cat) (GWB-374Z09) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for Haptoglobin has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and washed to remove unbound proteins. An Anti-Haptoglobin - HRP conjugated detector antibody is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Haptoglobin captured in the well.
Kit Component
ComponentAmount
Anti-Cat Haptoglobin Microplate96 Wells (12 x 8 Well Strips)
20X Diluent Concentrate50 mL
20X Wash Solution50 mL
100X Enzyme-Antibody Conjugate150 uL
Chromogen-Substrate Solution12 mL
Stop Solution12 mL
Cat Haptoglobin Calibrator1
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Homology Cat
Standard Range 400 ng/ml-6.25 ng/ml
Product Format In Solution
Specimen/Volumes Refer To Manual
Concentration Lot Specific
Application ELISA
Reconstitution and Storage 1. DILUENT
The 5X Diluent Concentrate is stable until the expira-tion date. The 1X working solution is stable for at least one week from the date of preparation. Both solutions should be stored at 4-8C.
2. WASH SOLUTION
The 20X Wash Solution Concentrate is stable until the expira¬tion date. The 1X working solution is stable for at least one week from the date of preparation. Both solutions can be stored at room temperature (16 25C) or at 4-8C.
3. ENZYME-ANTIBODY CONJUGATE
Undiluted horseradish peroxidase anti-Hp conjugate should be stored at 4-8C and diluted immediately prior to use. The working conjugate solution is stable for up to 8 hours.
4. CHROMOGEN-SUBSTRATE SOLUTION
The Substrate Solution should be stored at 4-8C and is stable until the expiration date.
5. STOP SOLUTION
The Stop Solution should be stored at 4-8C and is stable until the expiration date.
6. ANTI-CAT HAPTOGLOBIN ELISA MICRO PLATE
Anti-Cat Hp coated wells are stable until the expiration date, and should be stored at 4-8C in sealed foil pouch with desiccant pack.
7. CAT HAPTOGLOBIN STANDARDS
Long Term Storage: Upon receipt, aliquot the calibrator and store them frozen. They will be stable until expiration date. Short Term Storage: the calibrator is stable for up to 14 days at 4C. The working standard solutions should be prepared immediately prior to use and are stable for up to 8 hours.
Reactivity Cat
Kit Size 96 well
Kit Duration 70 Minutes
Datasheets/Manuals Printable datasheet for GWB-374Z09
Storage Refer to kit instructions for component storage
Species Reactivity Cat
Lead Time Domestic: within 1 week delivery | International: 1 week
Intended Use Research Use Only
Additional Information Intended Use: The Haptoglobin test kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Haptoglobin in serum or plasma of cats
:: Reagents:
1. DILUENT CONCENTRATE (Running Buffer)
One bottle containing 50 ml of a 5X concentrated diluent running buffer.
2. WASH SOLUTION CONCENTRATE
One bottle containing 50 ml of a 20X concentrated wash solution.
3. ENZYME-ANTIBODY CONJUGATE 100X
One vial containing 150 ul of affinity purified anti-Cat Haptoglobin antibody conjugated with horseradish peroxidase in a stabilizing buffer.
4. CHROMOGEN-SUBSTRATE SOLUTION
One vial containing 12 mL of 3,3’,5,5’-tetramethybenzidine (TMB) and hydrogen peroxide in citric acid buffer at pH 3.3.
5. STOP SOLUTION
One vial containing 12 ml 0.3 M sulfuric acid.
WARNING: Avoid contact with skin.
6. ANTI-CAT HAPTOGLOBIN ELISA MICRO PLATE
Twelve removable eight (8) well micro well strips in well holder frame. Each well is coated with affinity purified anti-Cat Hp.
7. CAT HAPTOGLOBIN CALIBRATOR
One vial containing a Cat Haptoglobin calibrator.