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96 Wells

Additional Information

Name FGFR3 ELISA Kit (Mouse) (GWB-KBBBI1)
Kit Range 0.156-10 ng/mL
Sensitivity <0.057ng/mL
Sample Type Serum, Plasma, Tissue homogenates, Cell lysates
Kit Detection Absorbance, OD 450 nm
Gene Symbol FGFR3
Gene id 14184
Alias Symbols CD333, Fgfr-3, FGFR-3, Fibroblast growth factor receptor 3, Flg-2, FR3, HBGFR, Heparin-binding growth factor receptor, Mfr3, sam3, Sam3
Swissprot ID Q61851
Protein Name Fibroblast growth factor receptor 3
Description of Target Tyrosine-protein kinase that acts as cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of cell proliferation, differentiation and apoptosis. plays an essential role in the regulation of chondrocyte different
Kit Principle Genway Biotech FGFR3 ELISA Kit (Mouse) (GWB-KBBBI1) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for FGFR3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for FGFR3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample FGFR3 captured in the well.
Kit Component
Anti-FGFR3 Microplate96 Wells (12 x 8 Well Strips)
FGFR3 Lyophilized Standard2 x 10 ng
Standard Diluent1 x 20 mL
100X FGFR3 Biotinylated Detector Antibody1 x 120 uL
100X Avidin-HRP Detection Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Detection Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3.5 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBBI1
Species Reactivity Mouse