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96 Wells

Additional Information

Name FGFRL1 ELISA Kit (Human) (GWB-KBBRP1)
Kit Range 0.156-10 ng/mL
Sensitivity < 0.051 ng/mL
Sample Type Serum, plasma, tissue homogenates
Kit Detection Absorbance, OD 450 nm
Gene Symbol FGFRL1
Gene id 53834
Alias Symbols FGF homologous factor receptor, FGFR5, FGFR-5, FGF receptor-like protein 1, FGFR-like protein, FHFR, Fibroblast growth factor receptor 5, Fibroblast growth factor receptor-like 1, UNQ480/PRO943
Swissprot ID Q8N441
Protein Name Fibroblast growth factor receptor-like 1
Description of Target The protein encoded by this gene is a member of the fibroblast growth factor receptor (FGFR) family, where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affini
Kit Principle Genway Biotech FGFRL1 ELISA Kit (Human) (GWB-KBBRP1) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for FGFRL1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for FGFRL1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample FGFRL1 captured in the well.
Kit Component
Anti-FGFRL1 Microplate96 Wells (12 x 8 Well Strips)
FGFRL1 Lyophilized Standard2 x 10 ng
Standard Diluent1 x 20 mL
100X FGFRL1 Biotinylated Detector Antibody1 x 120 µL
100X Avidin-HRP Detection Conjugate1 x 120 µL
Detector Antibody Diluent1 x 12 mL
Detection Conjugate Diluent1 x 12 mL
10X Sample Diluent1 x 30 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001004356.1
Nucleotide Accession Num NM_001004356.2
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3.5 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBRP1
Species Reactivity Human