FGL1 ELISA Kit (Human) (GWB-KBBMS7)

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GWB-KBBMS7

96 Wells
$659.00
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Additional Information

Name FGL1 ELISA Kit (Human) (GWB-KBBMS7)
Kit Range 0.156 - 10 ng/mL
Sensitivity < 0.074 ng/mL
Sample Type plasma
Kit Detection Colorimetric, OD 450 nm
Gene Symbol FGL1
Gene Fullname Fibrinogen like 1
Gene id 2267
Alias Symbols Fibrinogen-like protein 1, HP-041, Hepassocin, Hepatocyte-derived fibrinogen-related-protein 1, Liver fibrinogen-related protein 1, HFREP1, HP-041, LFIRE1, LFIRE-1
Swissprot ID Q08830
Protein Name Fibrinogen-like protein 1
Kit Principle Genway Biotech FGL1 ELISA Kit (Human) (GWB-KBBMS7) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for FGL1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for FGL1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample FGL1 captured in the well.
Kit Component
ComponentAmount
Anti-FGL1 Microplate96 Wells (12 x 8 Well Strips)
FGL1 Lyophilized Standard2 x 10 ng
100X Biotinylated FGL1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_004458.3
Nucleotide Accession Num NM_004467
Assay Info Assay Methodology: Quantitative Sandwich Immunoassay
Specificity This assay has high sensitivity and excellent specificity for detection of Fibrinogen Like Protein 1 (FGL1)._x000D_ No significant cross-reactivity or interference between Fibrinogen Like Protein 1 (FGL1) and analogues was observed.
Reconstitution and Storage Store as indicated in product manual.
Kit Duration ~ 3 Hours
Kit linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Sodium Citrate Plasma (n=5)99-105%97-105%96-105%96-103%
Kit Recovery
MatrixRecovery Range (%)Average (%)
Sodium Citrate Plasma (n=5)83-10193
Datasheets/Manuals Printable datasheet for GWB-KBBMS7
Species Reactivity Human