Fgl2 ELISA Kit (Rat) (GWB-KBBZZ9)

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96 Wells
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Additional Information

Name Fgl2 ELISA Kit (Rat) (GWB-KBBZZ9)
Kit Range 78.12 - 5,000 pg/mL
Sensitivity < 34 pg/mL
Sample Type plasma
Kit Detection Colorimetric, OD 450 nm
Gene Symbol FGL2
Gene id 84586
Alias Symbols T49, PT49, Fibroleukin
Swissprot ID Q32Q89
Protein Name Fgl2 protein
Description of Target structurally similar to the beta and gamma chains of fibrinogen; may by involved with physiological functions at mucosal sites; may play a role in the initiation of parturition [RGD, Feb 2006]
Kit Principle Genway Biotech Fgl2 ELISA Kit (Rat) (GWB-KBBZZ9) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Fgl2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Fgl2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Fgl2 captured in the well.
Kit Component
Anti-Atf4 Microplate96 Wells (12 x 8 Well Strips)
Atf4 Lyophilized Standard2 x 5 ng
100X Biotinylated Atf4 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_445907
Nucleotide Accession Num NM_053455
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Kit linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Sodium Citrate Plasma (n=5)82-102%87-102%78-90%90-99%
Kit Recovery
MatrixRecovery Range (%)Average (%)
Sodium Citrate Plasma (n=5)87-9692
Datasheets/Manuals Printable datasheet for GWB-KBBZZ9
Species Reactivity Rat