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96 Wells

Additional Information

Name FHL1 ELISA Kit (Human) (GWB-KBBCI6)
Kit Range 0.156-10 ng/mL
Sensitivity < 0.06 ng/mL
Sample Type Serum, Plasma, Tissue homogenates, Cell lysates
Kit Detection Absorbance, OD 450 nm
Gene Symbol FHL1
Gene id 2273
Alias Symbols bA535K18.1, FHL-1, FHL1A, FHL1B, FLH1A, Four and a half LIM domains protein 1, KYOT, KYO-T, MGC111107, Skeletal muscle LIM-protein 1, SLIM, SLIM1, SLIM-1, SLIMMER, XMPMA
Swissprot ID Q13642
Protein Name Four and a half LIM domains protein 1
Description of Target This gene encodes a member of the four-and-a-half-LIM-only protein family. Family members contain two highly conserved, tandemly arranged, zinc finger domains with four highly conserved cysteines binding a zinc atom in each zinc finger. Expression of thes
Kit Principle Genway Biotech FHL1 ELISA Kit (Human) (GWB-KBBCI6) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for FHL1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for FHL1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample FHL1 captured in the well.
Kit Component
Anti-FHL1 Microplate96 Wells (12 x 8 Well Strips)
FHL1 Lyophlized Standard2 x 10 ng
Standard Diluent1 x 20 mL
100X FHL1 Biotinylated Detector Antibody1 x 120 µL
100X Avidin-HRP Detection Conjugate1 x 120 µL
Detector Antibody Diluent1 x 12 mL
Detection Conjugate Diluent1 x 12 mL
10X Sample Diluent1 x 30 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001153171.1
Nucleotide Accession Num NM_001159699.1
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3.5 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBCI6
Species Reactivity Human