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96 Wells
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Additional Information

Name FLCN ELISA Kit (Human) (GWB-KBBTA2)
Kit Range 0.312 - 20 ng/mL
Sensitivity < 0.112 ng/mL
Sample Type tissue homogenates and other biological fluids
Kit Detection Colorimetric, OD 450 nm
Gene Symbol FLCN
Gene id 201163
Alias Symbols BHD, BHD skin lesion fibrofolliculoma protein, Birt-Hogg-Dube syndrome protein, DKFZp547A118, FLCL, FLJ45004, FLJ99377, Folliculin, MGC17998, MGC23445
Swissprot ID Q8NFG4
Protein Name Folliculin
Description of Target May be a tumor suppressor. May be involved in energy and/or nutrient sensing through the AMPK and mTOR signaling pathways. May regulate phosphorylation of RPS6KB1.
Kit Principle Genway Biotech FLCN ELISA Kit (Human) (GWB-KBBTA2) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for FLCN has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for FLCN is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample FLCN captured in the well.
Kit Component
Anti-FLCN Microplate96 Wells (12 x 8 Well Strips)
FLCN Lyophilized Standard2 x 20 ng
100X Biotinylated FLCN Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_653207
Nucleotide Accession Num NM_144606
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBTA2
Species Reactivity Human