Flt1 ELISA Kit (Pig) (GWB-KBBND7) (Aviva Cat. No. OKCD00849)

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Please see item number OKCD00849 to view product details and for ordering information.


GWB-KBBND7

Aviva Cat. No.: OKCD00849

Additional Information

Name Flt1 ELISA Kit (Pig) (GWB-KBBND7)
Kit Range 0.312 - 20 ng/mL
Sensitivity < 0.117 ng/mL
Sample Type serum, plasma, tissue homogenates and other biological fluids
Kit Detection Colorimetric, OD 450 nm
Gene Symbol FLT1
Gene id 54251
Alias Symbols Flt1, Fms-Related Tyrosine Kinase 1, Vascular Permeability Factor Receptor, Fms-like tyrosine kinase 1, Tyrosine-protein kinase receptor FLT
Swissprot ID P53767
Protein Name vascular endothelial growth factor receptor 1
Kit Principle Genway Biotech Flt1 ELISA Kit (Pig) (GWB-KBBND7) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Flt1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Flt1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Flt1 captured in the well.
Kit Component
ComponentAmount
Anti-NF2 Microplate96 Wells (12 x 8 Well Strips)
NF2 Lyophilized Standard2 x 20 ng
100X Biotinylated NF2 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001296310
Nucleotide Accession Num NM_001309381
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Kit linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)80-91%79-89%88-104%79-98%
EDTA Plasma (n=5)99-105%80-95%91-103%93-104%
Heparin Plasma (n=5)98-105%78-89%89-103%83-94%
Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)79-9893
EDTA Plasma (n=5)93-10599
Heparin Plasma (n=5)90-9794
Datasheets/Manuals Printable datasheet for GWB-KBBND7
Species Reactivity Pig