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Fth1 ELISA Kit (Mouse) (GWB-KBBRM5)

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GWB-KBBRM5

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Additional Information

Name Fth1 ELISA Kit (Mouse) (GWB-KBBRM5)
Description of Target Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Has ferroxidase activity. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells.
Kit Range 0.156 - 10 ng/mL
Sensitivity < 0.057 ng/mL
Sample Type serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Kit Detection Colorimetric, OD 450 nm
Gene Symbol FTH1
Gene id 14319
Alias Symbols AL022624, AL033366, Ferritin heavy chain, Ferritin H subunit, FHC, Fth, H-ferritin, HFt, MFH
Swissprot ID P09528
Protein Name ferritin heavy chain
Kit Principle Genway Biotech Fth1 ELISA Kit (Mouse) (GWB-KBBRM5) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Fth1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Fth1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Fth1 captured in the well.
Kit Component
ComponentAmount
Anti-NPR3 Microplate96 Wells (12 x 8 Well Strips)
NPR3 Lyophilized Standard2 x 10 ng
100X Biotinylated NPR3 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_034369
Nucleotide Accession Num NM_010239
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Kit linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)92-103%83-103%92-101%80-101%
EDTA Plasma (n=5)82-101%88-97%87-98%89-97%
Heparin Plasma (n=5)91-105%95-102%80-90%86-101%
Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)98-105101
EDTA Plasma (n=5)97-105101
Heparin Plasma (n=5)93-104101
Datasheets/Manuals Printable datasheet for GWB-KBBRM5
Species Reactivity Mouse