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Fxr1 ELISA Kit (Rat) (GWB-KBBEZ4)

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96 Wells
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Additional Information

Name Fxr1 ELISA Kit (Rat) (GWB-KBBEZ4)
Kit Range 0.156 - 10 ng/mL
Sensitivity < 0.053 ng/mL
Sample Type tissue homogenates, cell lysates and other biological fluids
Kit Detection Colorimetric, OD 450 nm
Gene Symbol FXR1
Gene id 361927
Alias Symbols Fragile X mental retardation syndrome-related protein 1, Fxr1h
Swissprot ID Q5XI81
Protein Name fragile X mental retardation syndrome-related protein 1
Description of Target RNA-binding protein required for embryonic and postnatal development of muscle tissue. May regulate intracellular transport and local translation of certain mRNAs .
Kit Principle Genway Biotech Fxr1 ELISA Kit (Rat) (GWB-KBBEZ4) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Fxr1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Fxr1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Fxr1 captured in the well.
Kit Component
Anti-XCL2 Microplate96 Wells (12 x 8 Well Strips)
XCL2 Lyophilized Standard2 x 10 ng
100X Biotinylated XCL2 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001012179
Nucleotide Accession Num NM_001012179
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBEZ4
Species Reactivity Rat