fragile X mental retardation syndrome-related protein 1
Description of Target
RNA-binding protein required for embryonic and postnatal development of muscle tissue. May regulate intracellular transport and local translation of certain mRNAs .
Kit Principle
Genway Biotech Fxr1 ELISA Kit (Rat) (GWB-KBBEZ4) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Fxr1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Fxr1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Fxr1 captured in the well.
