G6PC ELISA Kit (Human) (GWB-KBBBE9) (Aviva Cat. No. OKDD00277)

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GWB-KBBBE9

Aviva Cat. No.: OKDD00277

Additional Information

Name G6PC ELISA Kit (Human) (GWB-KBBBE9)
Kit Range 0.156-10 ng/mL
Sensitivity < 0.063 ng/mL
Sample Type Serum, Plasma, Tissue homogenates, Cell lysates
Kit Detection Absorbance, OD 450 nm
Gene Symbol G6PC
Gene id 2538
Alias Symbols G6Pase, G-6-Pase, G6Pase-alpha, G6PC1, G6PT, Glucose-6-phosphatase, Glucose-6-phosphatase alpha, GSD1, GSD1a, MGC163350
Swissprot ID P35575
Protein Name Glucose-6-phosphatase
Description of Target Glucose-6-phosphatase (G6Pase) is a multi-subunit integral membrane protein of the endoplasmic reticulum that is composed of a catalytic subunit and transporters for G6P, inorganic phosphate, and glucose. This gene (G6PC) is one of the three glucose-6-pho
Kit Principle Genway Biotech G6PC ELISA Kit (Human) (GWB-KBBBE9) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for G6PC has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for G6PC is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample G6PC captured in the well.
Kit Component
ComponentAmount
Anti-G6PC Microplate96 Wells (12 x 8 Well Strips)
G6PC Lyophlized Standard2 x 10 ng
Standard Diluent1 x 20 mL
100X G6PC Biotinylated Detector Antibody1 x 120 µL
100X Avidin-HRP Detection Conjugate1 x 120 µL
Detector Antibody Diluent1 x 12 mL
Detection Conjugate Diluent1 x 12 mL
10X Sample Diluent1 x 30 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_000142.2
Nucleotide Accession Num NM_000151.3
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3.5 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBBE9
Species Reactivity Human