| Name |
G6PC ELISA Kit (Human) (GWB-KBBBE9) |
| Kit Range |
0.156-10 ng/mL |
| Sensitivity |
< 0.063 ng/mL |
| Sample Type |
Serum, Plasma, Tissue homogenates, Cell lysates |
| Kit Detection |
Absorbance, OD 450 nm |
| Gene Symbol |
G6PC |
| Gene id |
2538 |
| Alias Symbols |
G6Pase, G-6-Pase, G6Pase-alpha, G6PC1, G6PT, Glucose-6-phosphatase, Glucose-6-phosphatase alpha, GSD1, GSD1a, MGC163350 |
| Swissprot ID |
P35575 |
| Protein Name |
Glucose-6-phosphatase |
| Description of Target |
Glucose-6-phosphatase (G6Pase) is a multi-subunit integral membrane protein of the endoplasmic reticulum that is composed of a catalytic subunit and transporters for G6P, inorganic phosphate, and glucose. This gene (G6PC) is one of the three glucose-6-pho |
| Kit Principle |
Genway Biotech G6PC ELISA Kit (Human) (GWB-KBBBE9) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for G6PC has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for G6PC is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample G6PC captured in the well. |
| Kit Component |
| Component | Amount |
| Anti-G6PC Microplate | 96 Wells (12 x 8 Well Strips) |
| G6PC Lyophlized Standard | 2 x 10 ng |
| Standard Diluent | 1 x 20 mL |
| 100X G6PC Biotinylated Detector Antibody | 1 x 120 µL |
| 100X Avidin-HRP Detection Conjugate | 1 x 120 µL |
| Detector Antibody Diluent | 1 x 12 mL |
| Detection Conjugate Diluent | 1 x 12 mL |
| 10X Sample Diluent | 1 x 30 mL |
| 30X Wash Buffer | 1 x 20 mL |
| TMB Substrate | 1 x 9 mL |
| Stop Solution | 1 x 6 mL | |
| Protein Accession Num |
NP_000142.2 |
| Nucleotide Accession Num |
NM_000151.3 |
| Assay Info |
Assay Methodology: Quantitative Sandwich ELISA |
| Reconstitution and Storage |
Store at 2 - 8C. Do not use the kit beyond the expiration date. |
| Kit Duration |
~ 3.5 Hours |
| Datasheets/Manuals |
Printable datasheet for GWB-KBBBE9 |
| Species Reactivity |
Human |
