G6pc ELISA Kit (Rat) (GWB-KBBUG1) (Aviva Cat. No. OKCD01980)

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Please see item number OKCD01980 to view product details and for ordering information.


GWB-KBBUG1

Aviva Cat. No.: OKCD01980

Additional Information

Name G6pc ELISA Kit (Rat) (GWB-KBBUG1)
Kit Range 1.56 - 100 ng/mL
Sensitivity < 0.59 ng/mL
Sample Type serum, plasma, tissue homogenates and other biological fluids
Kit Detection Colorimetric, OD 450 nm
Gene Symbol G6PC
Gene id 25634
Alias Symbols G6Pase, G-6-Pase, G6pt, Glucose-6-phosphatase, MGC93613
Swissprot ID P43428
Protein Name Glucose-6-phosphatase
Description of Target Hydrolyzes glucose-6-phosphate to glucose in the endoplasmic reticulum. Forms with the glucose-6-phosphate transporter (SLC37A4/G6PT) the complex responsible for glucose production through glycogenolysis and gluconeogenesis. Hence, it is the key enzyme in
Kit Principle Genway Biotech G6pc ELISA Kit (Rat) (GWB-KBBUG1) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for G6pc has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for G6pc is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample G6pc captured in the well.
Kit Component
ComponentAmount
Anti-G6pc Microplate96 Wells (12 x 8 Well Strips)
G6pc Lyophilized Standard2 x 100 ng
100X Biotinylated G6pc Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_037230
Nucleotide Accession Num NM_013098.
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Kit linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)87-101%90-99%80-104%92-101%
EDTA Plasma (n=5)86-104%80-93%80-94%78-92%
Heparin Plasma (n=5)93-105%85-99%78-103%78-91%
Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)98-105102
EDTA Plasma (n=5)87-9491
Heparin Plasma (n=5)78-8981
Datasheets/Manuals Printable datasheet for GWB-KBBUG1
Species Reactivity Rat