GADD45a ELISA Kit (Human) (GWB-KBBSA4)

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96 Wells

Additional Information

Name GADD45a ELISA Kit (Human) (GWB-KBBSA4)
Kit Range 0.156-10 ng/mL
Sensitivity < 0.064 ng/mL
Sample Type Serum, Plasma, Tissue homogenates, Cell lysates
Kit Detection Absorbance, OD 450 nm
Gene Symbol GADD45a
Gene id 1647
Alias Symbols DDIT1, DDIT-1, DNA damage-inducible transcript 1 protein, GADD45, Growth arrest and DNA damage-inducible protein GADD45 alpha
Swissprot ID P24522
Protein Name growth arrest and DNA damage-inducible protein GADD45 alpha
Description of Target This gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The protein encoded by this gene responds to environmental stresses by mediating activatio
Kit Principle Genway Biotech GADD45a ELISA Kit (Human) (GWB-KBBSA4) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for GADD45a has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for GADD45a is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample GADD45a captured in the well.
Kit Component
Anti-GADD45a Microplate96 Wells (12 x 8 Well Strips)
GADD45a Lyophlized Standard2 x 10 ng
Standard Diluent1 x 20 mL
100X GADD45a Biotinylated Detector Antibody1 x 120 µL
100X Avidin-HRP Detection Conjugate1 x 120 µL
Detector Antibody Diluent1 x 12 mL
Detection Conjugate Diluent1 x 12 mL
10X Sample Diluent1 x 30 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001186670.1
Nucleotide Accession Num NM_001199741.1
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3.5 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBSA4
Species Reactivity Human