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96 Wells
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Additional Information

Name GAN ELISA Kit (Human) (GWB-KBBUM9)
Kit Range 0.156 - 10 ng/mL
Sensitivity < 0.055 ng/mL
Sample Type tissue homogenates and other biological fluids.
Kit Detection Colorimetric, OD 450 nm
Gene Symbol GAN
Gene id 8139
Alias Symbols FLJ38059, GAN1, Gigaxonin, Kelch-like protein 16, KLHL16
Swissprot ID Q9H2C0
Protein Name gigaxonin
Description of Target Probable cytoskeletal component that directly or indirectly plays an important role in neurofilament architecture. May act as a substrate-specific adapter of an E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasom
Kit Principle Genway Biotech GAN ELISA Kit (Human) (GWB-KBBUM9) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for GAN has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for GAN is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample GAN captured in the well.
Kit Component
Anti-FAH Microplate96 Wells (12 x 8 Well Strips)
FAH Lyophilized Standard2 x 10 ng
100X Biotinylated FAH Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_071324
Nucleotide Accession Num NM_022041
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBUM9
Species Reactivity Human