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96 Wells
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Additional Information

Name GLDC ELISA Kit (Human) (GWB-KBBOW7)
Kit Range 0.312 - 20 ng/mL
Sensitivity < 0.132 ng/mL
Sample Type tissue homogenates, cell lysates and other biological fluids
Kit Detection Colorimetric, OD 450 nm
Gene Symbol GLDC
Gene id 2731
Alias Symbols GCE, GCSP, Glycine cleavage system P protein, Glycine decarboxylase, HYGN1, MGC138198, MGC138200, NKH
Swissprot ID P23378
Protein Name Glycine dehydrogenase (decarboxylating), mitochondrial
Description of Target The glycine cleavage system catalyzes the degradation of glycine. The P protein (GLDC) binds the alpha-amino group of glycine through its pyridoxal phosphate cofactor; CO2 is released and the remaining methylamine moiety is then transferred to the lipoami
Kit Principle Genway Biotech GLDC ELISA Kit (Human) (GWB-KBBOW7) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for GLDC has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for GLDC is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample GLDC captured in the well.
Kit Component
Anti-GLDC Microplate96 Wells (12 x 8 Well Strips)
GLDC Lyophilized Standard2 x 20 ng
100X Biotinylated GLDC Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_000161
Nucleotide Accession Num NM_000170
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBOW7
Species Reactivity Human