GNb1 ELISA Kit (Mouse) (GWB-KBBMP3)

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96 Wells

Additional Information

Name GNb1 ELISA Kit (Mouse) (GWB-KBBMP3)
Kit Range 0.156-10 ng/mL
Sensitivity <0.041 ng/mL
Sample Type Serum, plasma, tissue homogenates
Kit Detection Absorbance, OD 450 nm
Gene Symbol GNb1
Gene id 14688
Alias Symbols AA409223, C77571, Gnb-1, Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1, MGC98074, Transducin beta chain 1
Swissprot ID P62874
Protein Name guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1
Description of Target Guanine nucleotide-binding proteins (g proteins) are involved as a modulator or transducer in various transmembrane signaling systems. the beta and gamma chains are required for the gtpase activity, for replacement of gdp by gtp, and for g protein-effecto
Kit Principle Genway Biotech GNb1 ELISA Kit (Mouse) (GWB-KBBMP3) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for GNb1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for GNb1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample GNb1 captured in the well.
Kit Component
Anti-GNb1 Microplate96 Wells (12 x 8 Well Strips)
GNb1 Lyophilized Standard2 x 10 ng
Standard Diluent1 x 20 mL
100X GNb1 Biotinylated Detector Antibody1 x 120 uL
100X Avidin-HRP Detection Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Detection Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001153488.1
Nucleotide Accession Num NM_001160016.1
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3.5 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBMP3
Species Reactivity Mouse