Got1 ELISA Kit (Mouse) (GWB-KBBSK2)

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96 Wells
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Additional Information

Name Got1 ELISA Kit (Mouse) (GWB-KBBSK2)
Kit Range 0.312 - 20 ng/mL
Sensitivity < 0.114 ng/mL
Sample Type serum, plasma, tissue homogenates and other biological fluids
Kit Detection Colorimetric, OD 450 nm
Gene Symbol GOT1
Gene id 14718
Alias Symbols AI789014, Aspartate aminotransferase, cytoplasmic, cAspAT, cytosolic aspartate aminotransferase, Glutamate oxaloacetate transaminase 1, Got-1, Transaminase A
Swissprot ID P05201
Protein Name aspartate aminotransferase, cytoplasmic
Description of Target Biosynthesis of L-glutamate from L-aspartate or L-cysteine. Important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. Acts as a scavenger of glutamate in brain neuroprotection. The aspartat
Kit Principle Genway Biotech Got1 ELISA Kit (Mouse) (GWB-KBBSK2) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Got1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Got1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Got1 captured in the well.
Kit Component
Anti-Itga6 Microplate96 Wells (12 x 8 Well Strips)
Itga6 Lyophilized Standard2 x 20 ng
100X Biotinylated Itga6 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_034454
Nucleotide Accession Num NM_010324
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Kit linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)82-101%78-92%86-93%99-105%
EDTA Plasma (n=5)94-102%92-99%92-101%99-105%
Heparin Plasma (n=5)84-92%95-104%81-101%80-102%
Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)79-9087
EDTA Plasma (n=5)97-105101
Heparin Plasma (n=5)96-10499
Datasheets/Manuals Printable datasheet for GWB-KBBSK2
Species Reactivity Mouse