Gpc4 ELISA Kit (Rat) (GWB-KBBYO2)

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96 Wells
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Additional Information

Name Gpc4 ELISA Kit (Rat) (GWB-KBBYO2)
Kit Range 15.62 - 1,000 pg/mL
Sensitivity < 6.1 pg/mL
Sample Type serum, plasma, tissue homogenates and other biological fluids.
Kit Detection Colorimetric, OD 450 nm
Gene Symbol GPC4
Gene id 317322
Alias Symbols K-Glypican, Glypican Proteoglycan 4, Secreted glypican-4
Swissprot ID Q642B0
Protein Name glypican-4
Description of Target Cell surface proteoglycan that bears heparan sulfate.
Kit Principle Genway Biotech Gpc4 ELISA Kit (Rat) (GWB-KBBYO2) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Gpc4 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Gpc4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Gpc4 captured in the well.
Kit Component
Anti-Gpc4 Microplate96 Wells (12 x 8 Well Strips)
Gpc4 Lyophilized Standard2 x 1 ng
100X Biotinylated Gpc4 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001014130
Nucleotide Accession Num NM_001014108
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Kit linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)96-104%83-94%99-105%84-93%
EDTA Plasma (n=5)91-101%93-101%93-101%78-102%
Heparin Plasma (n=5)81-89%86-93%90-98%89-99%
Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)84-9389
EDTA Plasma (n=5)93-10198
Heparin Plasma (n=5)98-105102
Datasheets/Manuals Printable datasheet for GWB-KBBYO2
Species Reactivity Rat