GRIN2C ELISA Kit (Human) (GWB-KBBSV2)

This exact product is now provided by Aviva Systems Biology.
Please visit OKCD01843 to review further and for ordering information.


GWB-KBBSV2

96 Wells
$659.00
Add to Cart

Additional Information

Name GRIN2C ELISA Kit (Human) (GWB-KBBSV2)
Kit Range 0.312 - 20 ng/mL
Sensitivity < 0.118 ng/mL
Sample Type tissue homogenates and other biological fluids
Kit Detection Colorimetric, OD 450 nm
Gene Symbol GRIN2C
Gene id 2905
Alias Symbols NMDAR2C, N-methyl D-aspartate receptor subtype 2C, NR2C
Swissprot ID Q14957
Protein Name Glutamate receptor ionotropic, NMDA 2C
Description of Target NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine.
Kit Principle Genway Biotech GRIN2C ELISA Kit (Human) (GWB-KBBSV2) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for GRIN2C has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for GRIN2C is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample GRIN2C captured in the well.
Kit Component
ComponentAmount
Anti-GRIN2C Microplate96 Wells (12 x 8 Well Strips)
GRIN2C Lyophilized Standard2 x 20 ng
100X Biotinylated GRIN2C Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_000826
Nucleotide Accession Num NM_000835.
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBSV2
Species Reactivity Human