Product Datasheet: GWB-100A5F - Rat Anti-Mouse CD5/Lyt-1 Antibody (GWB-100A5F) - Genway Biotech
 
Rat Anti-Mouse CD5/Lyt-1 Antibody (GWB-100A5F)
Data Sheet
 
Product Number GWB-100A5F
Product Page www.genwaybio.com/rat-anti-mouse-cd5-lyt-1
Name Rat Anti-Mouse CD5/Lyt-1 Antibody (GWB-100A5F)
Reactivity Mouse
Size 100 ug
Related Product Names (Synonyms) Rat Anti-Mouse CD5/Ly-1-SPRD; N/A Rat Anti-Mouse CD5/Lyt-1CD5
Sequence Length 442
GI Number 458563
NCBI Acc Number AAA16552.1
Format *Spectral Red? (SPRD) Conjugate
Storage The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.0. No preservatives or amine-containing buffer salts added. Store at 2- 8C.
The fluorescein (FITC) conjugate is supplied as 0
Clonality Monoclonal
Intended Use Research Use Only
Characterization To ensure lot- to- lot consistency, each batch of monoclonal antibody is tested to conform withcharacteristics of a standard by flow cytometry. Representative data are included in this data sheet.
Clone 4H8E6
Official Gene Full Name T-cell surface glycoprotein CD5
NCBI Gene Id 921
Gene Symbol CD5
Host Rat
Isotype IgG2a
Product Format SpectralRed (SPRD) Conjugate
Dilution Flow Cytometry: Fluorescein conjugate <= 1 ug/106 cells
Biotin conjugate <= 1 ug/106 cells
R-phycoerythrin conjugate
Allophycocyanin conjugate
Spectral Red conjugate
<= 0.2 ug/106 cells
<= 0.2 ug/106 cells
<= 0.2 ug/106 cells
Target Reference 1. Ledbetter, J. A. and L. A. Herzenberg. 1979. Immunol. Rev. 47:63
2. Seaman, W. E., D. Wofsy, J. S. Greenspan, and J. A. Ledbetter. 1983. J. Immunol. 130:1713
3. Ledbetter, J. A., R. V. Rouse, H. S. Micklem, and L. A. Herzenberg. 1980. J. Exp. Med. 152:280
4. Van de Veld, H., I. von Hoegen, W. Luo, J. R. Parnes, and K. Thielemans. 1991. Nature 351:662
5. Luo, W., H. Van de Velde, I. von Hoegen, J. R. Parnes, and K. Thielemans. 1992. J. Immunol. 148:1630
6. Kantor, A. B. and L. A. Herzenberg. 1993. Ann. Rev. Immunol. 11:501
7. Holland, N. 1982. Immunol. Rev. 68:43
8. Stanton, T., T. L. Stevens, J. A. Ledbetter, and D. Wofsy. 1986. J. Immunol. 136:1734
9. Verwilghen J, R. Vandesande, P. Vandenberghe, J.L. Ceuppens . 1990. Cell Immunol. 131:109
10. Logdberg, L. and E. M. Shevach. 1996. Eur. J. Immunol. 15:1007
11. June, C. H., P. R. Rabinovitch, and J. A. Ledbetter. 1987. J. Immunol. 138:2782
12. Hollander, N., E. Pillemer, and I. L. Weissman. 1981. Proc. Natl. Acad. Sci. U. S. A. 78:1148
13. Tarakhovsky, A., S.B. Kanner, J. Hombach, J.A. ledbetter, W. Muller, N. Killeen, and K. Rajewsky. 1995. Science 269:535
14. Bikah, G., J. Carey, J.R. Cialella, A. Tarakhovsky, and S, Bobdada. 1996. Science 274:1906
15. Saunderson,S.C., P.C. Schuberth, A.C. Dunn, L. Miller, B.D. Hock, P.A. MacKay, N. Koch, R.W. Jack, and A.D. McLellan
2008. J. Immunol. 180:8146.
Lead Time Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Nucleotide Accession Num NM_014207.3
Protein Accession # NP_055022.2
Protein Name T-cell surface glycoprotein CD5
Protocol
The following related protocols are available on www.genwaybio.com
Replacement Item This antibody may replace item sc-1180 from Santa Cruz Biotechnology.
Specificity CD5/Lyt- 1, Mr 67 kDa
Swissprot Id P06127
Warning Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin,eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash withcopious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yieldstoxic hydrazoic acid under acidic conditions. Dilute azide- containing compounds in running water beforediscarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Datasheets/Manuals Printable datasheet for GWB-100A5F
Application Info - Identification and enumeration of CD5+ cells by flow cytometry 15
Notice This exact product is now provided by Aviva Systems Biology.
Please visit OASB00286 to review further and for ordering information.
Application Flow Cytometry (FACS), IP, IHC-F
Image 1
BALB/c spleen
Amount Used: ? 0.2 Ug/106 cells from BALB/c spleen were double-stained with rat anti-mouse CD5-R-PE and rat anti-mouse CD19-FITC, following which small lymphocytes were gated and analyzed using a FACScan flow cytometer (BDIS, San Jose, CA).
 

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