| Product Number |
GWB-100A5F |
| Product Page |
www.genwaybio.com/rat-anti-mouse-cd5-lyt-1 |
| Name |
Rat Anti-Mouse CD5/Lyt-1 Antibody (GWB-100A5F) |
| Reactivity |
Mouse |
| Size |
100 ug |
| Related Product Names (Synonyms) |
Rat Anti-Mouse CD5/Ly-1-SPRD; N/A Rat Anti-Mouse CD5/Lyt-1CD5 |
| Sequence Length |
442 |
| GI Number |
458563 |
| NCBI Acc Number |
AAA16552.1 |
| Format |
*Spectral Red? (SPRD) Conjugate |
| Storage |
The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.0. No preservatives or amine-containing buffer salts added. Store at 2- 8C.
The fluorescein (FITC) conjugate is supplied as 0 |
| Clonality |
Monoclonal |
| Intended Use |
Research Use Only |
| Characterization |
To ensure lot- to- lot consistency, each batch of monoclonal antibody is tested to conform withcharacteristics of a standard by flow cytometry. Representative data are included in this data sheet. |
| Clone |
4H8E6 |
| Official Gene Full Name |
T-cell surface glycoprotein CD5 |
| NCBI Gene Id |
921 |
| Gene Symbol |
CD5 |
| Host |
Rat |
| Isotype |
IgG2a |
| Product Format |
SpectralRed (SPRD) Conjugate |
| Dilution |
Flow Cytometry: Fluorescein conjugate <= 1 ug/106 cells
Biotin conjugate <= 1 ug/106 cells
R-phycoerythrin conjugate
Allophycocyanin conjugate
Spectral Red conjugate
<= 0.2 ug/106 cells
<= 0.2 ug/106 cells
<= 0.2 ug/106 cells |
| Target Reference |
1. Ledbetter, J. A. and L. A. Herzenberg. 1979. Immunol. Rev. 47:63
2. Seaman, W. E., D. Wofsy, J. S. Greenspan, and J. A. Ledbetter. 1983. J. Immunol. 130:1713
3. Ledbetter, J. A., R. V. Rouse, H. S. Micklem, and L. A. Herzenberg. 1980. J. Exp. Med. 152:280
4. Van de Veld, H., I. von Hoegen, W. Luo, J. R. Parnes, and K. Thielemans. 1991. Nature 351:662
5. Luo, W., H. Van de Velde, I. von Hoegen, J. R. Parnes, and K. Thielemans. 1992. J. Immunol. 148:1630
6. Kantor, A. B. and L. A. Herzenberg. 1993. Ann. Rev. Immunol. 11:501
7. Holland, N. 1982. Immunol. Rev. 68:43
8. Stanton, T., T. L. Stevens, J. A. Ledbetter, and D. Wofsy. 1986. J. Immunol. 136:1734
9. Verwilghen J, R. Vandesande, P. Vandenberghe, J.L. Ceuppens . 1990. Cell Immunol. 131:109
10. Logdberg, L. and E. M. Shevach. 1996. Eur. J. Immunol. 15:1007
11. June, C. H., P. R. Rabinovitch, and J. A. Ledbetter. 1987. J. Immunol. 138:2782
12. Hollander, N., E. Pillemer, and I. L. Weissman. 1981. Proc. Natl. Acad. Sci. U. S. A. 78:1148
13. Tarakhovsky, A., S.B. Kanner, J. Hombach, J.A. ledbetter, W. Muller, N. Killeen, and K. Rajewsky. 1995. Science 269:535
14. Bikah, G., J. Carey, J.R. Cialella, A. Tarakhovsky, and S, Bobdada. 1996. Science 274:1906
15. Saunderson,S.C., P.C. Schuberth, A.C. Dunn, L. Miller, B.D. Hock, P.A. MacKay, N. Koch, R.W. Jack, and A.D. McLellan
2008. J. Immunol. 180:8146. |
| Lead Time |
Domestic: within 1-2 weeks delivery | International: 1-2 weeks |
| Nucleotide Accession Num |
NM_014207.3 |
| Protein Accession # |
NP_055022.2 |
| Protein Name |
T-cell surface glycoprotein CD5 |
| Protocol |
The following related protocols are available on www.genwaybio.com |
| Replacement Item |
This antibody may replace item sc-1180 from Santa Cruz Biotechnology. |
| Specificity |
CD5/Lyt- 1, Mr 67 kDa |
| Swissprot Id |
P06127 |
| Warning |
Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin,eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash withcopious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yieldstoxic hydrazoic acid under acidic conditions. Dilute azide- containing compounds in running water beforediscarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing. |
| Datasheets/Manuals |
Printable datasheet for GWB-100A5F |
| Application Info |
- Identification and enumeration of CD5+ cells by flow cytometry 15 |
| Notice |
This exact product is now provided by Aviva Systems Biology.
Please visit OASB00286 to review further and for ordering information. |
| Application |
Flow Cytometry (FACS), IP, IHC-F |
| Image 1 | BALB/c spleen
 | Amount Used: ? 0.2 Ug/106 cells from BALB/c spleen were double-stained with rat anti-mouse CD5-R-PE and rat anti-mouse CD19-FITC, following which small lymphocytes were gated and analyzed using a FACScan flow cytometer (BDIS, San Jose, CA). |
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