||mTOR / FRAP Antibody (GWB-31014E)
||Human, Mouse, Rat
|Related Product Names (Synonyms)
||mTOR / FRAP Rabbit Monoclonal Antibody FRAP1, FRAP, FRAP2, Serine/threonine-protein kinase mTOR, FKBP12-rapamycin complex-associated protein;FK506-binding protein 12-rmTOR / FRAP
||Store at -20C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
||Research Use Only
||Clone ID: Y391
Immunogen: A synthetic peptide corresponding to residues near the C-term of PI3K/PI4K domain of human mTOR/FRAP was used as an immunogen Specificity: A synthetic peptide corresponding to residues near the C-term of PI3K/PI4K domain of human mTOR/FRAP was used as an immunogen Dilution: WB: 1:1,000-2,000
IP: 1:50. The mammalian target of rapamycin (mTOR) is a major effector of cell growth and proliferation via the regulation of protein synthesis. mTOR is composed of an N-term; 20 tandem repeats- HEAT which are implicated in protein-protein interactions and a C-term; which includes a FAT domain, a FBR domain, a kinase domain, a NDR domain and a FATC domain. The FATC domain is essential to mTOR activity and the deletion of a single amino acid from this domain abrogates the activity. mTOR can be auto-phosphorylated via its intrinsic serine/threonine kinase activity. mTOR regulates protein synthesis through the phosphorylation and inactivation of the repressor of mRNA translation 4E-BP1 and though the phosphorylation and activation of S6 kinase. RAPTOR (regulatory associated protein of TOR) is a positive regulator of TOR. Other known mediators of mTOR include PI3-K and ATK from the insulin pathway. 1. Xiao L, Wang YC, Li WS, et al, The role of mTOR and phospho-p70S6K in pathogenesis and progression of gastric carcinomas: an immunohistochemical study on tissue microarray ,J Exp Clin Cancer Res 28 (2009) : 152
1. Hay, N.; Sonenberg, N. :Genes Dev. 18: 1926-1945, 2004.
2. Manufactured under U.S. Patents, No. 5,675,063 and 7,429,487
||Printable datasheet for GWB-31014E
||WB, IHC, ICC, FC, IP