Product Datasheet: GWB-374Z16 - Canine Alpha 1-Antitrypsin ELISA (GWB-374Z16) - Genway Biotech
 
Canine Alpha 1-Antitrypsin ELISA (GWB-374Z16)
Data Sheet
 
Product Number GWB-374Z16
Product Page www.genwaybio.com/canine-alpha-1-antitrypsin-elisa
Name Canine Alpha 1-Antitrypsin ELISA (GWB-374Z16)
Reactivity Canine
Size #N/A
Concentration Lot Specific
Related Product Names (Synonyms) A1AT, AAT, Alpha-1-antitrypsin, Alpha-1 protease inhibitor, Alpha-1-antiproteinase, Serpin A, SERPINA1, PI, Dog A1AT ELISA, Canine A1AT ELISA, Dog AAT ELISA, Canine AAT ELISA Kit
Storage Refer to kit instructions for component storage
Kit Size 96 well
Standard Range 800 ng/ml-12.5 ng/ml
Sample Type Serum, plasma and other biological fluids
Specimen/Volumes Refer To Manual
Intended Use Research Use Only
Alias Symbols SERPINA1, AAT, A1AT, alpha1AT
Assay Info Assay Methodology: Quantitative Sandwich ELISA
NCBI Gene Id 480422
Product Format In Solution
Sensitivity 3.823 ng/ml
Additional Information Intended Use: The Alpha 1-Antitrypsin (A1AT) test kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring A1AT in biological fluid of dogs.
Additional Information Reagents:
1. DILUENT CONCENTRATE (Running Buffer)
One bottle containing 50 ml of a 5X concentrated diluent running buffer.
2. WASH SOLUTION CONCENTRATE
One bottle containing 50 ml of a 20X concentrated wash solution.
3. ENZYME-ANTIBODY CONJUGATE 100X
One vial containing 150 ul of affinity purified anti-Dog Alpha 1-Antitrypsin antibody conjugated with horseradish peroxidase in a stabilizing buffer.
4. CHROMOGEN-SUBSTRATE SOLUTION
One vial containing 12 mL of 3,3’,5,5’-tetramethybenzidine (TMB) and hydrogen peroxide in citric acid buffer at pH 3.3.
5. STOP SOLUTION
One vial containing 12 ml 0.3 M sulfuric acid.
WARNING: Avoid contact with skin.
6. ANTI-DOG ALPHA 1-ANTITRYPSIN ELISA MICRO PLATE
Twelve removable eight (8) well micro well strips in well holder frame. Each well is coated with affinity purified anti-Dog A1AT.
7. DOG ALPHA 1-ANTITRYPSIN CALIBRATOR
One vial containing a Dog Alpha 1-Antitrypsin calibrator.
Kit Component
ComponentAmount
Anti-Dog Alpha 1-Antitrypsin Microplate96 Wells (12 x 8 Well Strips)
20X Diluent Concentrate50 mL
20X Wash Solution50 mL
100X Enzyme-Antibody Conjugate150 uL
Chromogen-Substrate Solution12 mL
Stop Solution12 mL
Dog Alpha 1-Antitrypsin Calibrator1
Kit Duration 70 Minutes
Kit Principle Genway Biotech Alpha 1-Antitrypsin ELISA Kit (Dog) (GWB-374Z16) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for SERPINA1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and washed to remove unbound proteins. An Anti-SERPINA1 - HRP conjugated detector antibody is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample SERPINA1 captured in the well.
Kit Range 12.5 ng/ml - 800 ng/ml
Lead Time Domestic: within 1 week delivery | International: 1 week
Protein Name SERPINA1
Reconstitution and Storage 1. DILUENT
The 5X Diluent Concentrate is stable until the expira-tion date. The 1X working solution is stable for at least one week from the date of preparation. Both solutions should be stored at 4-8C.
2. WASH SOLUTION
The 20X Wash Solution Concentrate is stable until the expira¬tion date. The 1X working solution is stable for at least one week from the date of preparation. Both solutions can be stored at room temperature (16 25C) or at 4-8C.
3. ENZYME-ANTIBODY CONJUGATE
Undiluted horseradish peroxidase anti-A1AT conjugate should be stored at 4-8C and diluted immediately prior to use. The working conjugate solution is stable for up to 8 hours.
4. CHROMOGEN-SUBSTRATE SOLUTION
The Substrate Solution should be stored at 4-8C and is stable until the expiration date.
5. STOP SOLUTION
The Stop Solution should be stored at 4-8C and is stable until the expiration date.
6. ANTI-DOG ALPHA 1-ANTITRYPSIN ELISA MICRO PLATE
Anti-Dog A1AT coated wells are stable until the expiration date, and should be stored at 4-8C in sealed foil pouch with desiccant pack.
7. DOG ALPHA 1-ANTITRYPSIN CALIBRATOR
Long Term Storage: Upon receipt, aliquot the calibrator and store them frozen. They will be stable until expiration date. Short Term Storage: the calibrator is stable for up to 14 days at 4C. The working standard solutions should be prepared immediately prior to use and are stable for up to 8 hours.
Swissprot Id F1PCE5
Datasheets/Manuals Printable datasheet for GWB-374Z16
Notice This exact product is available to purchase through Aviva Systems Biology.
Please see item number OKIA00022 to view product details and for ordering information.
Species Reactivity Dog
Predicted Homology Based on Immunogen Sequence Dog
Application ELISA
Image 1
Standard curve
Sample Standard Curve is for demonstration only. Do not use for results calculations. A standard curve should be generated each time the test is performed.
 

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