| Product Number |
GWB-5428A0 |
| Product Page |
www.genwaybio.com/human-alpha-1-antitrypsin-elisa-kit |
| Name |
Human Alpha 1-Antitrypsin ELISA Kit (GWB-5428A0) |
| Reactivity |
Human |
| Size |
96 Wells |
| Related Product Names (Synonyms) |
Alpha-1 protease inhibitor, Alpha-1-antiproteinase, Human alpha 1-Antitrypsin ELISA Kit; Alpha-1 protease inhibitor, Alpha-1-antiproteinase, AAT, PI Human alpha 1 Antitrypsin, SERPINA1, PI, AAT ELISA, A1AT, Human Alpha 1-Antitrypsin ELISA Kit |
| Storage |
Refer to kit instructions for component storage |
| Kit Size |
96 well |
| Standard Range |
Refer To Manual |
| Sample Type |
Serum, plasma and other biological fluids |
| Specimen/Volumes |
Refer To Manual |
| Intended Use |
Research Use Only |
| Alias Symbols |
SERPINA1, AAT, A1AT, alpha1AT |
| Assay Info |
Assay Methodology: Quantitative Sandwich ELISA |
| Official Gene Full Name |
serpin family A member 1 |
| NCBI Gene Id |
5265 |
| Gene Symbol |
SERPINA1 |
| Product Format |
In Solution |
| Sensitivity |
1.094 ng/ml |
| Additional Information |
Intended Use: The Alpha 1-Antitrypsin (A1AT) test kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring A1AT in biological fluid of humans. |
| Additional Information |
Reagents:
1. DILUENT CONCENTRATE (Running Buffer)
One bottle containing 50 ml of a 5X concentrated diluent running buffer.
2. WASH SOLUTION CONCENTRATE
One bottle containing 50 ml of a 20X concentrated wash solution.
3. ENZYME-ANTIBODY CONJUGATE 100X
One vial containing 150 ul of affinity purified anti-Human Alpha 1-Antitrypsin antibody conjugated with horseradish peroxidase in a stabilizing buffer.
4. CHROMOGEN-SUBSTRATE SOLUTION
One vial containing 12 mL of 3,3,5,5-tetramethybenzidine (TMB) and hydrogen peroxide in citric acid buffer at pH 3.3.
5. STOP SOLUTION
One vial containing 12 ml 0.3 M sulfuric acid.
WARNING: Avoid contact with skin.
6. ANTI-HUMAN ALPHA 1-ANTITRYPSIN ELISA MICRO PLATE
Twelve removable eight (8) well micro well strips in well holder frame. Each well is coated with affinity purified anti-Human A1AT.
7. HUMAN ALPHA 1-ANTITRYPSIN CALIBRATOR
One vial containing a lyophilized Human Alpha 1-Antitrypsin calibrator. |
| Kit Component |
| Component | Amount |
|---|
| Anti-Human Alpha 1-Antitrypsin Microplate | 96 Wells (12 x 8 Well Strips) |
| 20X Diluent Concentrate | 50 mL |
| 20X Wash Solution | 50 mL |
| 100X Enzyme-Antibody Conjugate | 150 uL |
| Chromogen-Substrate Solution | 12 mL |
| Stop Solution | 12 mL |
| Human Alpha 1-Antitrypsin Calibrator | 1 |
|
| Kit Duration |
100 Minutes |
| Kit Principle |
Genway Biotech Alpha 1-Antitrypsin ELISA Kit (Human) (GWB-5428A0) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for Alpha-1-antitrypsin has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and washed to remove unbound proteins. An Anti-Alpha-1-antitrypsin - HRP conjugated detector antibody is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Alpha-1-antitrypsin captured in the well. |
| Kit Range |
7.81 ng/ml - 500 ng/ml |
| Lead Time |
Domestic: within 1 week delivery | International: 1 week |
| Protein Name |
Alpha-1-antitrypsin |
| Reconstitution and Storage |
1. DILUENT
The 5X Diluent Concentrate is stable until the expira-tion date. The 1X working solution is stable for at least one week from the date of preparation. Both solutions should be stored at 4-8C.
2. WASH SOLUTION
The 20X Wash Solution Concentrate is stable until the expira¬tion date. The 1X working solution is stable for at least one week from the date of preparation. Both solutions can be stored at room temperature (16 25C) or at 4-8C.
3. ENZYME-ANTIBODY CONJUGATE
Undiluted horseradish peroxidase anti-A1AT conjugate should be stored at 4-8C and diluted immediately prior to use. The working conjugate solution is stable for up to 8 hours.
4. CHROMOGEN-SUBSTRATE SOLUTION
The Substrate Solution should be stored at 4-8C and is stable until the expiration date.
5. STOP SOLUTION
The Stop Solution should be stored at 4-8C and is stable until the expiration date.
6. ANTI-HUMAN ALPHA 1-ANTITRYPSIN ELISA MICRO PLATE
Anti-Human A1AT coated wells are stable until the expiration date, and should be stored at 4-8C in sealed foil pouch with desiccant pack.
7. HUMAN ALPHA 1-ANTITRYPSIN CALIBRATOR
The lyophilized Human Alpha 1-Antitrypsin calibrator should be stored at 4C or frozen until reconstituted. The reconstituted calibrator should be aliquoted out and stored frozen (Avoid multiple freeze-thaw cycles). The working standard solutions should be prepared immediately prior to use and are stable for up to 8 hours. |
| Swissprot Id |
P01009 |
| Datasheets/Manuals |
Printable datasheet for GWB-5428A0 |
| Notice |
This exact product is now provided by Aviva Systems Biology.
Please visit OKIA00048 to review further and for ordering information. |
| Description of Target |
Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in |
| Species Reactivity |
Human |
| Predicted Homology Based on Immunogen Sequence |
Human |
| Application |
ELISA |
| Image 1 | Standard curve
 | Sample Standard Curve is for demonstration only. Do not use for results calculations. A standard curve should be generated each time the test is performed. |
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