Product Name: TBE/FSME IgG ELISA
# of Samples: 1 x 96 Assays
Intended Use: The TBE/FSME IgG-ELISA is intended for the quantitative determination of IgG class antibodies against TBE virus in human serum or plasma (citrate).
Introduction: Tick-borne encephalitis (TBE) virus is a flavivirus of the family Togaviridae. It is an enveloped single-stranded RNA virus with cubic icosahedral symmetry and ranges in size from 20-80nm in diameter.
On the European continent only two antigenic subtypes exist which show only little differences in their structural proteins.
TBE virus is mainly transmitted by ticks. The degree of contamination of ticks (and thus humans) in central Europe increases from west to east, and anybody may be affected. Specific antibody development yields a life-long immunity.
TBE is the most important tick-transmitted disease of man -beside Lyme disease, which is caused by the spirochete Borrelia burgdorferi. The clinical course of the disease depends on the immune status of the infected persons. A high virus production in the primary infected tissues is required for the passage of the blood-brain barrier and the resulting severe manifestations in the central nervous system.
Of the two subtypes, RSSE causes the more severe infection, having an incidence of mortality of up to 25% in some outbreaks, whereas mortality in CEE seldom exceeds 5%. An inactivated TBE virus vaccine is available in Europe and Russia. The presence of virus resp. infection may be identified by:
Haemagglutination-Inhibition, Complement Fixation
Detection of specific antibodies by CIE, ELISA
Principles of the assay: The quantitative immunoenzymatic determination of IgG-class antibodies against TBE Virus is based on the ELISA (Enzyme-linked Immunosorbent Assay) technique.
Microtiterstrip wells are precoated with TBE Virus antigens to bind corresponding antibodies of the specimen. After washing the wells to remove all unbound sample material horseradish peroxidase (HRP) labelled anti-human IgG conjugate is added. This conjugate binds to the captured TBE Virus specific antibodies. The immune complex formed by the bound conjugate is visualized by adding Tetramethylbenzidine (TMB) substrate which gives a blue reaction product.
The intensity of this product is proportional to the amount of TBE Virus specific IgG antibodies in the specimen. Sulphuric acid is added to stop the reaction. This produces a yellow endpoint colour. Absorbance at 450 nm is read using an ELISA microwell plate reader.
Storage and Stability: The reagents are stable up to the expiry date stated on the label when stored at 2...8 °C.
References: Roggendorf M. Frühsommer-Meningoenzephalitis Wer soll geimpft werden? Therapiewoche, 40, 1173 (1990)Roggendorf M. et al. Serological Diagnosis of Acute Tick-Borne Encephalitis by Demonstration of Antibodies of the IgM class. J.Med.Virol., 7, 41 (1981)Hofmann H. et al. Rapid diagnosis of Tick-Borne Encephalitis by Means of Enzyme Linked Immunosorbent Assay. J.Gen.Virol. , 42, 505 (1979)Hofmann H. et al. ELISA for IgM Antibodies against Tick-Borne Encephalitis Virus: Quantification and Standardization of Results. Zbl.Bakt.I.Orig., 255, 448 (1983)Tick-Borne Encephalitis (TBE) and its Immunoprophylaxis, IMMUNO AG, Wien (1997)