Product Datasheet: GWB-F4BE8D - Human Ferritin ELISA Kit (GWB-F4BE8D) - Genway Biotech
 
Human Ferritin ELISA Kit (GWB-F4BE8D)
Data Sheet
 
Product Number GWB-F4BE8D
Product Page www.genwaybio.com/human-ferritin-elisa-kit
Name Human Ferritin ELISA Kit (GWB-F4BE8D)
Reactivity Human
Size 96 Wells
Related Product Names (Synonyms) Human Ferritin ELISA Kit
Storage Refer to kit instructions for component storage
Kit Size 96 well
Standard Range Refer To Manual
Sample Type Serum, plasma and other biological fluids
Specimen/Volumes Refer To Manual
Intended Use Research Use Only
Alias Symbols FTL, Ferritin light chain, FTL1
Official Gene Full Name ferritin light chain
NCBI Gene Id 2512
Gene Symbol FTL
Product Format In Solution
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Sensitivity 0.745 ng/ml
Replacement Aviva Sku OKIA00061
Additional Information Intended Use: The Ferritin test kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Ferritin in serum or plasma samples of humans.
Additional Information Reagents:
1. DILUENT CONCENTRATE (Running Buffer)
One bottle containing 50 ml of a 5X concentrated diluent running buffer.
2. WASH SOLUTION CONCENTRATE
One bottle containing 50 ml of a 20X concentrated wash solution.
3. ENZYME-ANTIBODY CONJUGATE 100X
One vial containing 150 ul of affinity purified anti-Human Ferritin antibody conjugated with horseradish peroxidase in a stabilizing buffer.
4. CHROMOGEN-SUBSTRATE SOLUTION
One vial containing 12 mL of 3,3’,5,5’-tetramethybenzidine (TMB) and hydrogen peroxide in citric acid buffer at pH 3.3.
5. STOP SOLUTION
One vial containing 12 ml 0.3 M sulfuric acid.
WARNING: Avoid contact with skin.
6. ANTI-HUMAN FERRITIN ELISA MICRO PLATE
Twelve removable eight (8) well micro well strips in well holder frame. Each well is coated with affinity purified anti-Human Ferritin.
7. HUMAN FERRITIN CALIBRATOR
One vial containing 1.0 ml of Human Ferritin Calibrator.
Kit Component
ComponentAmount
Anti-Human Ferritin Microplate96 Wells (12 x 8 Well Strips)
20X Diluent Concentrate50 mL
20X Wash Solution50 mL
100X Enzyme-Antibody Conjugate150 uL
Chromogen-Substrate Solution12 mL
Stop Solution12 mL
Human Ferritin Calibrator1
Kit Duration 80 Minutes
Kit Principle Genway Biotech Ferritin ELISA Kit (Human) (GWB-F4BE8D) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for Ferritin light chain has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and washed to remove unbound proteins. An Anti-Ferritin light chain - HRP conjugated detector antibody is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Ferritin light chain captured in the well.
Kit Range 1.56 ng/ml - 100 ng/ml
Lead Time Domestic: within 1 week delivery | International: 1 week
Protein Name Ferritin light chain
Reconstitution and Storage 1. DILUENT
The 5X Diluent Concentrate is stable until the expira-tion date. The 1X working solution is stable for at least one week from the date of preparation. Both solutions should be stored at 4-8C.
2. WASH SOLUTION
The 20X Wash Solution Concentrate is stable until the expira¬tion date. The 1X working solution is stable for at least one week from the date of preparation. Both solutions can be stored at room temperature (16 25C) or at 4-8C.
3. ENZYME-ANTIBODY CONJUGATE
Undiluted horseradish peroxidase anti-Ferritin conjugate should be stored at 4-8C and diluted immediately prior to use. The working conjugate solution is stable for up to 8 hours.
4. CHROMOGEN-SUBSTRATE SOLUTION
The Substrate Solution should be stored at 4-8C and is stable until the expiration date.
5. STOP SOLUTION
The Stop Solution should be stored at 4-8C and is stable until the expiration date.
6. ANTI-HUMAN FERRITIN ELISA MICRO PLATE
Anti-Human Ferritin coated wells are stable until the expiration date, and should be stored at 4-8C in sealed foil pouch with desiccant pack.
7. HUMAN FERRITIN CALIBRATOR
Long Term Storage: Upon receipt, aliquot the calibrator and store them frozen. They will be stable until expiration date. Short Term Storage: the calibrator is stable for up to 14 days at 4C. The working standard solutions should be prepared immediately prior to use and are stable for up to 8 hours.
Swissprot Id P02792
Datasheets/Manuals Printable datasheet for GWB-F4BE8D
Notice This exact product is available to purchase through Aviva Systems Biology.
Please see item number OKIA00061 to view product details and for ordering information.
Description of Target Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in c
Replacement Aviva Sku Url https://www.avivasysbio.com/ferritin-elisa-kit-human-96-wells-okia00061.html
Species Reactivity Human
Predicted Homology Based on Immunogen Sequence Human
Application ELISA
Image 1
Standard curve
Sample Standard Curve is for demonstration only. Do not use for results calculations. A standard curve should be generated each time the test is performed.
 

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