Product Datasheet: GWB-KBBEA4 - LACRT ELISA Kit (Human) (GWB-KBBEA4) - Genway Biotech
Data Sheet
Product Number GWB-KBBEA4
Product Page
Size 96 Wells
Sample Type saliva, tears and other biological fluids
Alias Symbols Extracellular glycoprotein lacritin, LACRITIN
Assay Info Assay Methodology: Quantitative Sandwich ELISA
NCBI Gene Id 90070
Gene Symbol LACRT
Protein Name extracellular glycoprotein lacritin
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Sensitivity < 0.58 ng/mL
Kit Component
Anti-RPN1 Microplate96 Wells (12 x 8 Well Strips)
RPN1 Lyophilized Standard2 x 100 ng
100X Biotinylated RPN1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Kit Detection Method Colorimetric, OD 450 nm
Kit Duration ~ 3 Hours
Kit Principle Genway Biotech LACRT ELISA Kit (Human) (GWB-KBBEA4) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for LACRT has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for LACRT is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample LACRT captured in the well.
Kit Range 1.56 - 100 ng/mL
Nucleotide Accession Num NM_033277
Protein Accession # NP_150593
Swissprot Id Q9GZZ8
Datasheets/Manuals Printable datasheet for GWB-KBBEA4
Notice This exact product is now provided by Aviva Systems Biology.
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Description of Target Modulates secretion by lacrimal acinar cells.
Species Reactivity Human
Image 1
Standard Curve
LACRT ELISA Kit (Human) (GWB-KBBEA4) standard curve.

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