||LACRT ELISA Kit (Human) (GWB-KBBEA4)
||saliva, tears and other biological fluids
||Extracellular glycoprotein lacritin, LACRITIN
||Assay Methodology: Quantitative Sandwich ELISA
|NCBI Gene Id
||extracellular glycoprotein lacritin
|Reconstitution and Storage
||Store at 2 - 8C. Do not use the kit beyond the expiration date.
||< 0.58 ng/mL
|Anti-RPN1 Microplate||96 Wells (12 x 8 Well Strips)|
|RPN1 Lyophilized Standard||2 x 100 ng|
|100X Biotinylated RPN1 Detector Antibody||120 uL|
|Avidin/HRP Conjugate||120 uL|
|Standard Diluent||1 x 20 mL|
|Detector Antibody Diluent||1 x 12 mL|
|Conjugate Diluent||1 x 12 mL|
|30X Wash Buffer||1 x 20 mL|
|TMB Substrate||1 x 9 mL|
|Stop Solution||1 x 6 mL|
|Kit Detection Method
||Colorimetric, OD 450 nm
||~ 3 Hours
||Genway Biotech LACRT ELISA Kit (Human) (GWB-KBBEA4) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for LACRT has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for LACRT is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample LACRT captured in the well.
||1.56 - 100 ng/mL
|Nucleotide Accession Num
|Protein Accession #
||Printable datasheet for GWB-KBBEA4
||This exact product is now provided by Aviva Systems Biology.
Please visit OKCD00685 to review further and for ordering information.
|Description of Target
||Modulates secretion by lacrimal acinar cells.
|Standard Curve||LACRT ELISA Kit (Human) (GWB-KBBEA4) standard curve.|