Product Datasheet: GWB-KBBHS9 - Serpinc1 ELISA Kit (Rat) (GWB-KBBHS9) - Genway Biotech
 
Serpinc1 ELISA Kit (Rat) (GWB-KBBHS9)
Data Sheet
 
Product Number GWB-KBBHS9
Product Page www.genwaybio.com/serpinc1-elisa-kit-rat-gwb-kbbhs9
Name Serpinc1 ELISA Kit (Rat) (GWB-KBBHS9)
Size 96 Wells
Sample Type serum, plasma and other biological fluids.
Alias Symbols AT3, ATIII, SERPINC1, Anti-Thrombin Antibodies, Serpin Peptidase Inhibitor Clade C Member 1, Coding Sequence Signal Peptide Antithrombin Part 1
Assay Info Assay Methodology: Quantitative Sandwich ELISA
NCBI Gene Id 304917
Gene Symbol SERPINC1
Protein Name antithrombin-III
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Sensitivity < 1.38 ng/mL
Kit Component
ComponentAmount
Anti-Pcsk1 Microplate96 Wells (12 x 8 Well Strips)
Pcsk1 Lyophilized Standard2 x 200 ng
100X Biotinylated Pcsk1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Kit Detection Method Colorimetric, OD 450 nm
Kit Duration ~ 3 Hours
Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)87-94%83-90%92-105%92-105%
EDTA Plasma (n=5)89-96%81-103%83-99%93-101%
Heparin Plasma (n=5)94-102%99-105%90-102%92-105%
Kit Principle Genway Biotech Serpinc1 ELISA Kit (Rat) (GWB-KBBHS9) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Serpinc1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Serpinc1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Serpinc1 captured in the well.
Kit Range 3.12 - 200 ng/mL
Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)80-9380
EDTA Plasma (n=5)79-9995
Heparin Plasma (n=5)98-105102
Nucleotide Accession Num NM_080844
Protein Accession # NP_543120
Swissprot Id Q5M7T5
Datasheets/Manuals Printable datasheet for GWB-KBBHS9
Notice This exact product is now provided by Aviva Systems Biology.
Please visit OKCD01116 to review further and for ordering information.
Species Reactivity Rat
Image 1
Standard Curve
Serpinc1 ELISA Kit (Rat) (GWB-KBBHS9) standard curve.
 

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