||CFHR5 ELISA Kit (Human) (GWB-KBBIK2)
||serum, plasma and other biological fluids
||CFHL5, Complement factor H-related protein 5, FHR5, FHR-5, FLJ10549, MGC133240
||Assay Methodology: Quantitative Sandwich ELISA
|NCBI Gene Id
||Complement factor H-related protein 5
|Reconstitution and Storage
||Store at 2 - 8C. Do not use the kit beyond the expiration date.
||< 14.8 pg/mL
|Anti-CFHR5 Microplate||96 Wells (12 x 8 Well Strips)|
|CFHR5 Lyophilized Standard||2 x 2 ng|
|100X Biotinylated CFHR5 Detector Antibody||120 uL|
|Avidin/HRP Conjugate||120 uL|
|Standard Diluent||1 x 20 mL|
|Detector Antibody Diluent||1 x 12 mL|
|Conjugate Diluent||1 x 12 mL|
|30X Wash Buffer||1 x 20 mL|
|TMB Substrate||1 x 9 mL|
|Stop Solution||1 x 6 mL|
|Kit Detection Method
||Colorimetric, OD 450 nm
||~ 3 Hours
|Sample||1:2 Dilution||1:4 Dilution||1:8 Dilution||1:16 Dilution|
|EDTA Plasma (n=5)||83-101%||81-102%||98-105%||89-96%|
|Heparin Plasma (n=5)||97-104%||92-101%||79-94%||89-97%|
||Genway Biotech CFHR5 ELISA Kit (Human) (GWB-KBBIK2) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CFHR5 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CFHR5 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CFHR5 captured in the well.
||31.2 - 2,000 pg/mL
|Matrix||Recovery Range (%)||Average (%)|
|EDTA Plasma (n=5)||88-99||95|
|Heparin Plasma (n=5)||80-95||88|
|Nucleotide Accession Num
|Protein Accession #
||Printable datasheet for GWB-KBBIK2
||This exact product is now provided by Aviva Systems Biology.
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|Description of Target
||Involved in complement regulation. The dimerized forms have avidity for tissue-bound complement fragments and efficiently compete with the physiological complement inhibitor CFH.
|Standard Curve||CFHR5 ELISA Kit (Human) (GWB-KBBIK2) standard curve.|