Product Datasheet: GWB-KBBIK2 - CFHR5 ELISA Kit (Human) (GWB-KBBIK2) - Genway Biotech
Data Sheet
Product Number GWB-KBBIK2
Product Page
Name CFHR5 ELISA Kit (Human) (GWB-KBBIK2)
Size 96 Wells
Sample Type serum, plasma and other biological fluids
Alias Symbols CFHL5, Complement factor H-related protein 5, FHR5, FHR-5, FLJ10549, MGC133240
Assay Info Assay Methodology: Quantitative Sandwich ELISA
NCBI Gene Id 81494
Gene Symbol CFHR5
Protein Name Complement factor H-related protein 5
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Sensitivity < 14.8 pg/mL
Kit Component
Anti-CFHR5 Microplate96 Wells (12 x 8 Well Strips)
CFHR5 Lyophilized Standard2 x 2 ng
100X Biotinylated CFHR5 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Kit Detection Method Colorimetric, OD 450 nm
Kit Duration ~ 3 Hours
Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)78-104%79-97%78-93%78-103%
EDTA Plasma (n=5)83-101%81-102%98-105%89-96%
Heparin Plasma (n=5)97-104%92-101%79-94%89-97%
Kit Principle Genway Biotech CFHR5 ELISA Kit (Human) (GWB-KBBIK2) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CFHR5 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CFHR5 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CFHR5 captured in the well.
Kit Range 31.2 - 2,000 pg/mL
Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)88-103101
EDTA Plasma (n=5)88-9995
Heparin Plasma (n=5)80-9588
Nucleotide Accession Num NM_030787.
Protein Accession # NP_110414
Swissprot Id Q9BXR6
Datasheets/Manuals Printable datasheet for GWB-KBBIK2
Notice This exact product is now provided by Aviva Systems Biology.
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Description of Target Involved in complement regulation. The dimerized forms have avidity for tissue-bound complement fragments and efficiently compete with the physiological complement inhibitor CFH.
Species Reactivity Human
Image 1
Standard Curve
CFHR5 ELISA Kit (Human) (GWB-KBBIK2) standard curve.

Genway Biotech manufactures and sells quality antibody products covering genome wide proteins.

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