||Cxcl8 ELISA Kit (Monkey) (GWB-KBBIW5)
||serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
||CXCL8, AMCF-I, GCP1, K60, LECT, LUCT, LYNAP, MDNCF, MONAP, NAF, NAP1, SCYB8, TSG1, B-ENAP, Neutrophil-Activating Protein 1, Granulocyte Chemotactic Protein 1
||Assay Methodology: Quantitative Sandwich ELISA
|NCBI Gene Id
|Reconstitution and Storage
||Store at 2 - 8C. Do not use the kit beyond the expiration date.
||< 5.5 pg/mL
|Anti-CXCL8 Microplate||96 Wells (12 x 8 Well Strips)|
|CXCL8 Lyophilized Standard||2 x 1 ng|
|100X Biotinylated CXCL8 Detector Antibody||120 uL|
|Avidin/HRP Conjugate||120 uL|
|Standard Diluent||1 x 20 mL|
|Detector Antibody Diluent||1 x 12 mL|
|Conjugate Diluent||1 x 12 mL|
|30X Wash Buffer||1 x 20 mL|
|TMB Substrate||1 x 9 mL|
|Stop Solution||1 x 6 mL|
|Kit Detection Method
||Colorimetric, OD 450 nm
||~ 3 Hours
|Sample||1:2 Dilution||1:4 Dilution||1:8 Dilution||1:16 Dilution|
|EDTA Plasma (n=5)||93-101%||82-94%||91-98%||79-95%|
|Heparin Plasma (n=5)||81-94%||95-102%||94-102%||85-94%|
||Genway Biotech Cxcl8 ELISA Kit (Monkey) (GWB-KBBIW5) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Cxcl8 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Cxcl8 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Cxcl8 captured in the well.
||15.62 - 1,000 pg/mL
|Matrix||Recovery Range (%)||Average (%)|
|EDTA Plasma (n=5)||93-101||96|
|Heparin Plasma (n=5)||93-102||99|
|Nucleotide Accession Num
|Protein Accession #
||Printable datasheet for GWB-KBBIW5
||This exact product is available to purchase through Aviva Systems Biology.
Please see item number OKCD00142 to view product details and for ordering information.
|Description of Target
||IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus (
|Standard Curve||Cxcl8 ELISA Kit (Monkey) (GWB-KBBIW5) standard curve.|