Product Datasheet: GWB-KBBIW5 - Cxcl8 ELISA Kit (Monkey) (GWB-KBBIW5) - Genway Biotech
 
Cxcl8 ELISA Kit (Monkey) (GWB-KBBIW5)
Data Sheet
 
Product Number GWB-KBBIW5
Product Page www.genwaybio.com/cxcl8-elisa-kit-monkey-gwb-kbbiw5
Name Cxcl8 ELISA Kit (Monkey) (GWB-KBBIW5)
Size 96 Wells
Sample Type serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Alias Symbols CXCL8, AMCF-I, GCP1, K60, LECT, LUCT, LYNAP, MDNCF, MONAP, NAF, NAP1, SCYB8, TSG1, B-ENAP, Neutrophil-Activating Protein 1, Granulocyte Chemotactic Protein 1
Assay Info Assay Methodology: Quantitative Sandwich ELISA
NCBI Gene Id 613028
Gene Symbol CXCL8
Protein Name interleukin-8
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Sensitivity < 5.5 pg/mL
Kit Component
ComponentAmount
Anti-CXCL8 Microplate96 Wells (12 x 8 Well Strips)
CXCL8 Lyophilized Standard2 x 1 ng
100X Biotinylated CXCL8 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Kit Detection Method Colorimetric, OD 450 nm
Kit Duration ~ 3 Hours
Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)88-97%96-103%85-96%95-102%
EDTA Plasma (n=5)93-101%82-94%91-98%79-95%
Heparin Plasma (n=5)81-94%95-102%94-102%85-94%
Kit Principle Genway Biotech Cxcl8 ELISA Kit (Monkey) (GWB-KBBIW5) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Cxcl8 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Cxcl8 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Cxcl8 captured in the well.
Kit Range 15.62 - 1,000 pg/mL
Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)80-8882
EDTA Plasma (n=5)93-10196
Heparin Plasma (n=5)93-10299
Nucleotide Accession Num NM_001032965
Protein Accession # NP_001028137
Swissprot Id P67813
Datasheets/Manuals Printable datasheet for GWB-KBBIW5
Notice This exact product is available to purchase through Aviva Systems Biology.
Please see item number OKCD00142 to view product details and for ordering information.
Description of Target IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus (
Species Reactivity Monkey
Image 1
Standard Curve
Cxcl8 ELISA Kit (Monkey) (GWB-KBBIW5) standard curve.
 

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