| Product Number |
GWB-KBBIW5 |
| Product Page |
www.genwaybio.com/cxcl8-elisa-kit-monkey-gwb-kbbiw5 |
| Name |
Cxcl8 ELISA Kit (Monkey) (GWB-KBBIW5) |
| Size |
96 Wells |
| Sample Type |
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. |
| Alias Symbols |
CXCL8, AMCF-I, GCP1, K60, LECT, LUCT, LYNAP, MDNCF, MONAP, NAF, NAP1, SCYB8, TSG1, B-ENAP, Neutrophil-Activating Protein 1, Granulocyte Chemotactic Protein 1 |
| Assay Info |
Assay Methodology: Quantitative Sandwich ELISA |
| NCBI Gene Id |
613028 |
| Gene Symbol |
CXCL8 |
| Protein Name |
interleukin-8 |
| Reconstitution and Storage |
Store at 2 - 8C. Do not use the kit beyond the expiration date. |
| Sensitivity |
< 5.5 pg/mL |
| Kit Component |
| Component | Amount |
|---|
| Anti-CXCL8 Microplate | 96 Wells (12 x 8 Well Strips) | | CXCL8 Lyophilized Standard | 2 x 1 ng | | 100X Biotinylated CXCL8 Detector Antibody | 120 uL | | Avidin/HRP Conjugate | 120 uL | | Standard Diluent | 1 x 20 mL | | Detector Antibody Diluent | 1 x 12 mL | | Conjugate Diluent | 1 x 12 mL | | 30X Wash Buffer | 1 x 20 mL | | TMB Substrate | 1 x 9 mL | | Stop Solution | 1 x 6 mL |
|
| Kit Detection Method |
Colorimetric, OD 450 nm |
| Kit Duration |
~ 3 Hours |
| Kit Linearity |
| Sample | 1:2 Dilution | 1:4 Dilution | 1:8 Dilution | 1:16 Dilution |
|---|
| Serum (n=5) | 88-97% | 96-103% | 85-96% | 95-102% | |
| EDTA Plasma (n=5) | 93-101% | 82-94% | 91-98% | 79-95% | |
| Heparin Plasma (n=5) | 81-94% | 95-102% | 94-102% | 85-94% | |
|
| Kit Principle |
Genway Biotech Cxcl8 ELISA Kit (Monkey) (GWB-KBBIW5) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Cxcl8 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Cxcl8 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Cxcl8 captured in the well. |
| Kit Range |
15.62 - 1,000 pg/mL |
| Kit Recovery |
| Matrix | Recovery Range (%) | Average (%) |
|---|
| Serum (n=5) | 80-88 | 82 | |
| EDTA Plasma (n=5) | 93-101 | 96 | |
| Heparin Plasma (n=5) | 93-102 | 99 | |
|
| Nucleotide Accession Num |
NM_001032965 |
| Protein Accession # |
NP_001028137 |
| Swissprot Id |
P67813 |
| Datasheets/Manuals |
Printable datasheet for GWB-KBBIW5 |
| Notice |
This exact product is available to purchase through Aviva Systems Biology.
Please see item number OKCD00142 to view product details and for ordering information. |
| Description of Target |
IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus ( |
| Species Reactivity |
Monkey |
| Image 1 | Standard Curve
 | Cxcl8 ELISA Kit (Monkey) (GWB-KBBIW5) standard curve. |
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