Product Datasheet: GWB-KBBLO7 - Serpinf1 ELISA Kit (Rat) (GWB-KBBLO7) - Genway Biotech
 
Serpinf1 ELISA Kit (Rat) (GWB-KBBLO7)
Data Sheet
 
Product Number GWB-KBBLO7
Product Page www.genwaybio.com/serpinf1-elisa-kit-rat-gwb-kbblo7
Name Serpinf1 ELISA Kit (Rat) (GWB-KBBLO7)
Size 96 Wells
Sample Type serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Alias Symbols Dmrs91, Pedf
Assay Info Assay Methodology: Quantitative Sandwich ELISA
NCBI Gene Id 287526
Gene Symbol SERPINF1
Protein Name pigment epithelium-derived factor
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Sensitivity < 0.059 ng/mL
Kit Component
ComponentAmount
Anti-SLC7A5 Microplate96 Wells (12 x 8 Well Strips)
SLC7A5 Lyophilized Standard2 x 10 ng
100X Biotinylated SLC7A5 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Kit Detection Method Colorimetric, OD 450 nm
Kit Duration ~ 3 Hours
Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)84-99%86-93%96-105%93-104%
EDTA Plasma (n=5)91-103%89-101%82-93%95-105%
Heparin Plasma (n=5)88-101%87-96%87-94%78-102%
Kit Principle Genway Biotech Serpinf1 ELISA Kit (Rat) (GWB-KBBLO7) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Serpinf1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Serpinf1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Serpinf1 captured in the well.
Kit Range 0.156 - 10 ng/mL
Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)78-9383
EDTA Plasma (n=5)87-10197
Heparin Plasma (n=5)88-9992
Nucleotide Accession Num NM_177927
Protein Accession # NP_808788
Swissprot Id Q80ZA3
Datasheets/Manuals Printable datasheet for GWB-KBBLO7
Notice This exact product is now provided by Aviva Systems Biology.
Please visit OKCD00297 to review further and for ordering information.
Species Reactivity Rat
Image 1
Standard Curve
Serpinf1 ELISA Kit (Rat) (GWB-KBBLO7) standard curve.
 

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