Product Datasheet: GWB-KBBLZ4 - CFHR3 ELISA Kit (Human) (GWB-KBBLZ4) - Genway Biotech
 
CFHR3 ELISA Kit (Human) (GWB-KBBLZ4)
Data Sheet
 
Product Number GWB-KBBLZ4
Product Page www.genwaybio.com/cfhr3-elisa-kit-human-gwb-kbblz4
Name CFHR3 ELISA Kit (Human) (GWB-KBBLZ4)
Size 96 Wells
Sample Type Serum, Plasma
Alias Symbols CFHL3, Complement factor H-related protein 3, DOWN16, FHR3, FHR-3, H factor-like protein 3, HLF4
Assay Info Assay Methodology: Quantitative Sandwich ELISA
NCBI Gene Id 10878
Gene Symbol CFHR3
Protein Name Complement factor H-related protein 3
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Sensitivity < 0.057 ng/mL
Kit Component
ComponentAmount
Anti-CFHR3 Microplate96 Wells (12 x 8 Well Strips)
CFHR3 Lyophlized Standard2 x 10 ng
Standard Diluent1 x 20 mL
100X CFHR3 Biotinylated Detector Antibody1 x 120 µL
100X Avidin-HRP Detection Conjugate1 x 120 µL
Detector Antibody Diluent1 x 12 mL
Detection Conjugate Diluent1 x 12 mL
10X Sample Diluent1 x 30 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Kit Detection Method Absorbance, OD 450 nm
Kit Duration ~ 3.5 Hours
Kit Principle Genway Biotech CFHR3 ELISA Kit (Human) (GWB-KBBLZ4) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CFHR3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CFHR3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CFHR3 captured in the well.
Kit Range 0.156-10 ng/mL
Nucleotide Accession Num NM_001166624.1
Protein Accession # NP_001160096.1
Swissprot Id Q02985
Datasheets/Manuals Printable datasheet for GWB-KBBLZ4
Notice This exact product is now provided by Aviva Systems Biology.
Please visit OKDD00185 to review further and for ordering information.
Description of Target The protein encoded by this gene is a secreted protein, which belongs to the complement factor H-related protein family. It binds to heparin, and may be involved in complement regulation. Mutations in this gene are associated with decreased risk of age-re
Species Reactivity Human
Image 1
Standard Curve
CFHR3 ELISA Kit (Human) (GWB-KBBLZ4) Standard Curve
 

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