||CFHR3 ELISA Kit (Human) (GWB-KBBLZ4)
||CFHL3, Complement factor H-related protein 3, DOWN16, FHR3, FHR-3, H factor-like protein 3, HLF4
||Assay Methodology: Quantitative Sandwich ELISA
|NCBI Gene Id
||Complement factor H-related protein 3
|Reconstitution and Storage
||Store at 2 - 8C. Do not use the kit beyond the expiration date.
||< 0.057 ng/mL
|Anti-CFHR3 Microplate||96 Wells (12 x 8 Well Strips)|
|CFHR3 Lyophlized Standard||2 x 10 ng|
|Standard Diluent||1 x 20 mL|
|100X CFHR3 Biotinylated Detector Antibody||1 x 120 µL|
|100X Avidin-HRP Detection Conjugate||1 x 120 µL|
|Detector Antibody Diluent||1 x 12 mL|
|Detection Conjugate Diluent||1 x 12 mL|
|10X Sample Diluent||1 x 30 mL|
|30X Wash Buffer||1 x 20 mL|
|TMB Substrate||1 x 9 mL|
|Stop Solution||1 x 6 mL|
|Kit Detection Method
||Absorbance, OD 450 nm
||~ 3.5 Hours
||Genway Biotech CFHR3 ELISA Kit (Human) (GWB-KBBLZ4) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CFHR3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CFHR3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CFHR3 captured in the well.
|Nucleotide Accession Num
|Protein Accession #
||Printable datasheet for GWB-KBBLZ4
||This exact product is now provided by Aviva Systems Biology.
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|Description of Target
||The protein encoded by this gene is a secreted protein, which belongs to the complement factor H-related protein family. It binds to heparin, and may be involved in complement regulation. Mutations in this gene are associated with decreased risk of age-re
|Standard Curve||CFHR3 ELISA Kit (Human) (GWB-KBBLZ4) Standard Curve|