Product Datasheet: GWB-KBBMS7 - FGL1 ELISA Kit (Human) (GWB-KBBMS7) - Genway Biotech
 
FGL1 ELISA Kit (Human) (GWB-KBBMS7)
Data Sheet
 
Product Number GWB-KBBMS7
Product Page www.genwaybio.com/fgl1-elisa-kit-human-gwb-kbbms7
Name FGL1 ELISA Kit (Human) (GWB-KBBMS7)
Size 96 Wells
Sample Type plasma
Alias Symbols Fibrinogen-like protein 1, HP-041, Hepassocin, Hepatocyte-derived fibrinogen-related-protein 1, Liver fibrinogen-related protein 1, HFREP1, HP-041, LFIRE1, LFIRE-1
Assay Info Assay Methodology: Quantitative Sandwich Immunoassay
Official Gene Full Name Fibrinogen like 1
NCBI Gene Id 2267
Gene Symbol FGL1
Nucleotide Accession Num NM_004467.3
Protein Name Fibrinogen-like protein 1
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Sensitivity < 0.074 ng/mL
Replacement Aviva Sku OKCD01734
Kit Component
ComponentAmount
Anti-FGL1 Microplate96 Wells (12 x 8 Well Strips)
FGL1 Lyophilized Standard2 x 10 ng
100X Biotinylated FGL1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Kit Detection Method Colorimetric, OD 450 nm
Kit Duration ~ 3 Hours
Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Sodium Citrate Plasma (n=5)99-105%97-105%96-105%96-103%
Kit Principle Genway Biotech FGL1 ELISA Kit (Human) (GWB-KBBMS7) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for FGL1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for FGL1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample FGL1 captured in the well.
Kit Range 0.156 - 10 ng/mL
Kit Recovery
MatrixRecovery Range (%)Average (%)
Sodium Citrate Plasma (n=5)83-10193
Nucleotide Accession Num NM_004467
Protein Accession # NP_004458.3
Reconstitution and Storage Store as indicated in product manual.
Specificity This assay has high sensitivity and excellent specificity for detection of Fibrinogen Like Protein 1 (FGL1)._x000D_ No significant cross-reactivity or interference between Fibrinogen Like Protein 1 (FGL1) and analogues was observed.
Swissprot Id Q08830
Datasheets/Manuals Printable datasheet for GWB-KBBMS7
Notice This exact product is available to purchase through Aviva Systems Biology.
Please see item number OKCD01734 to view product details and for ordering information.
Replacement Aviva Sku Url https://www.avivasysbio.com/fgl1-elisa-kit-human-okcd01734.html
Species Reactivity Human
Image 1
FGL1 ELISA Standard Curve
Human FGL1 ELISA Kit Standard Curve
 

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