| Product Number |
GWB-KBBOA5 |
| Product Page |
www.genwaybio.com/serpini1-elisa-kit-human-gwb-kbboa5 |
| Name |
SERPINI1 ELISA Kit (Human) (GWB-KBBOA5) |
| Size |
96 Wells |
| Sample Type |
serum, plasma and other biological fluids |
| Alias Symbols |
DKFZp781N13156, neuroserpin, Neuroserpin, Peptidase inhibitor 12, PI12, PI-12, Serpin I1 |
| Assay Info |
Assay Methodology: Quantitative Sandwich ELISA |
| NCBI Gene Id |
5274 |
| Gene Symbol |
SERPINI1 |
| Protein Name |
neuroserpin |
| Reconstitution and Storage |
Store at 2 - 8C. Do not use the kit beyond the expiration date. |
| Sensitivity |
< 0.34 ng/mL |
| Kit Component |
| Component | Amount |
|---|
| Anti-Irs2 Microplate | 96 Wells (12 x 8 Well Strips) | | Irs2 Lyophilized Standard | 2 x 50 ng | | 100X Biotinylated Irs2 Detector Antibody | 120 uL | | Avidin/HRP Conjugate | 120 uL | | Standard Diluent | 1 x 20 mL | | Detector Antibody Diluent | 1 x 12 mL | | Conjugate Diluent | 1 x 12 mL | | 30X Wash Buffer | 1 x 20 mL | | TMB Substrate | 1 x 9 mL | | Stop Solution | 1 x 6 mL |
|
| Kit Detection Method |
Colorimetric, OD 450 nm |
| Kit Duration |
~ 3 Hours |
| Kit Linearity |
| Sample | 1:2 Dilution | 1:4 Dilution | 1:8 Dilution | 1:16 Dilution |
|---|
| Serum (n=5) | 83-103% | 94-105% | 81-96% | 78-98% | |
| EDTA Plasma (n=5) | 79-102% | 88-102% | 89-102% | 85-93% | |
| Heparin Plasma (n=5) | 95-102% | 97-105% | 97-104% | 86-97% | |
|
| Kit Principle |
Genway Biotech SERPINI1 ELISA Kit (Human) (GWB-KBBOA5) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SERPINI1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SERPINI1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample SERPINI1 captured in the well. |
| Kit Range |
0.781 - 50 ng/mL |
| Kit Recovery |
| Matrix | Recovery Range (%) | Average (%) |
|---|
| Serum (n=5) | 91-105 | 96 | |
| EDTA Plasma (n=5) | 88-95 | 91 | |
| Heparin Plasma (n=5) | 92-105 | 99 | |
|
| Nucleotide Accession Num |
NM_001122752 |
| Protein Accession # |
NP_001116224 |
| Swissprot Id |
Q99574 |
| Datasheets/Manuals |
Printable datasheet for GWB-KBBOA5 |
| Notice |
This exact product is now provided by Aviva Systems Biology.
Please visit OKCD01254 to review further and for ordering information. |
| Description of Target |
Serine protease inhibitor that inhibits plasminogen activators and plasmin but not thrombin. May be involved in the formation or reorganization of synaptic connections as well as for synaptic plasticity in the adult nervous system. May protect neurons fro |
| Species Reactivity |
Human |
| Image 1 | Standard Curve
 | SERPINI1 ELISA Kit (Human) (GWB-KBBOA5) standard curve. |
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