Product Datasheet: GWB-KBBOR3 - COCH ELISA Kit (Human) (GWB-KBBOR3) - Genway Biotech
 
COCH ELISA Kit (Human) (GWB-KBBOR3)
Data Sheet
 
Product Number GWB-KBBOR3
Product Page www.genwaybio.com/coch-elisa-kit-human-gwb-kbbor3
Name COCH ELISA Kit (Human) (GWB-KBBOR3)
Size 96 Wells
Sample Type tissue homogenates, cell lysates and other biological fluids
Alias Symbols COCH5B2, COCH-5B2, Cochlin, DFNA9, UNQ257/PRO294
Assay Info Assay Methodology: Quantitative Sandwich ELISA
NCBI Gene Id 1690
Gene Symbol COCH
Protein Name Cochlin
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Sensitivity < 0.057 ng/mL
Kit Component
ComponentAmount
Anti-COCH Microplate96 Wells (12 x 8 Well Strips)
COCH Lyophilized Standard2 x 10 ng
100X Biotinylated COCH Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Kit Detection Method Colorimetric, OD 450 nm
Kit Duration ~ 3 Hours
Kit Principle Genway Biotech COCH ELISA Kit (Human) (GWB-KBBOR3) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for COCH has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for COCH is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample COCH captured in the well.
Kit Range 0.156 - 10 ng/mL
Nucleotide Accession Num NM_001135058
Protein Accession # NP_001128530
Swissprot Id O43405
Datasheets/Manuals Printable datasheet for GWB-KBBOR3
Notice This exact product is available to purchase through Aviva Systems Biology.
Please see item number OKCD02001 to view product details and for ordering information.
Description of Target Plays a role in the control of cell shape and motility in the trabecular meshwork.
Species Reactivity Human
Image 1
Standard Curve
COCH ELISA Kit (Human) (GWB-KBBOR3) standard curve.
 

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