||30K, Calcium-activated neutral proteinase small subunit, Calcium-dependent protease small subunit, Calcium-dependent protease small subunit 1, CALPAIN4, Calpain regulatory subunit, Calpain small subunit 1, CANP, CANPS, CANP small subunit, CAPN4, CAPNS, CD
||Assay Methodology: Quantitative Sandwich ELISA
|NCBI Gene Id
||CAPNS1 ELISA Kit (Human) (GWB-KBBOY9)
||calpain small subunit 1
|Reconstitution and Storage
||Store at 2 - 8C. Do not use the kit beyond the expiration date.
||tissue homogenates and other biological fluids.
||< 0.126 ng/mL
|Replacement Aviva Sku
|Anti-Crybb1 Microplate||96 Wells (12 x 8 Well Strips)|
|Crybb1 Lyophilized Standard||2 x 20 ng|
|100X Biotinylated Crybb1 Detector Antibody||120 uL|
|Avidin/HRP Conjugate||120 uL|
|Standard Diluent||1 x 20 mL|
|Detector Antibody Diluent||1 x 12 mL|
|Conjugate Diluent||1 x 12 mL|
|30X Wash Buffer||1 x 20 mL|
|TMB Substrate||1 x 9 mL|
|Stop Solution||1 x 6 mL|
|Kit Detection Method
||Colorimetric, OD 450 nm
||~ 3 Hours
||Genway Biotech CAPNS1 ELISA Kit (Human) (GWB-KBBOY9) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CAPNS1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CAPNS1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CAPNS1 captured in the well.
||0.312 - 20 ng/mL
|Nucleotide Accession Num
|Protein Accession #
||Printable datasheet for GWB-KBBOY9
||This exact product is available to purchase through Aviva Systems Biology.
Please see item number OKCD00965 to view product details and for ordering information.
|Description of Target
||Regulatory subunit of the calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.
|Replacement Aviva Sku Url
|Standard Curve||CAPNS1 ELISA Kit (Human) (GWB-KBBOY9) standard curve.|