Product Datasheet: GWB-KBBOY9 - CAPNS1 ELISA Kit (Human) (GWB-KBBOY9) - Genway Biotech
 
CAPNS1 ELISA Kit (Human) (GWB-KBBOY9)
Data Sheet
 
Product Number GWB-KBBOY9
Product Page www.genwaybio.com/capns1-elisa-kit-human-gwb-kbboy9
Alias Symbols 30K, Calcium-activated neutral proteinase small subunit, Calcium-dependent protease small subunit, Calcium-dependent protease small subunit 1, CALPAIN4, Calpain regulatory subunit, Calpain small subunit 1, CANP, CANPS, CANP small subunit, CAPN4, CAPNS, CD
Assay Info Assay Methodology: Quantitative Sandwich ELISA
NCBI Gene Id 826
Gene Symbol CAPNS1
Name CAPNS1 ELISA Kit (Human) (GWB-KBBOY9)
Protein Name calpain small subunit 1
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Sample Type tissue homogenates and other biological fluids.
Sensitivity < 0.126 ng/mL
Size 96 Wells
Replacement Aviva Sku OKCD00965
Kit Component
ComponentAmount
Anti-Crybb1 Microplate96 Wells (12 x 8 Well Strips)
Crybb1 Lyophilized Standard2 x 20 ng
100X Biotinylated Crybb1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Kit Detection Method Colorimetric, OD 450 nm
Kit Duration ~ 3 Hours
Kit Principle Genway Biotech CAPNS1 ELISA Kit (Human) (GWB-KBBOY9) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CAPNS1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CAPNS1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CAPNS1 captured in the well.
Kit Range 0.312 - 20 ng/mL
Nucleotide Accession Num NM_001003962
Protein Accession # NP_001003962
Swissprot Id P04632
Datasheets/Manuals Printable datasheet for GWB-KBBOY9
Notice This exact product is available to purchase through Aviva Systems Biology.
Please see item number OKCD00965 to view product details and for ordering information.
Description of Target Regulatory subunit of the calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.
Replacement Aviva Sku Url https://www.avivasysbio.com/capns1-elisa-kit-human-okcd00965.html
Species Reactivity Human
Image 1
Standard Curve
CAPNS1 ELISA Kit (Human) (GWB-KBBOY9) standard curve.
 

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