Product Datasheet: GWB-KBBPX3 - CHGB ELISA Kit (Human) (GWB-KBBPX3) - Genway Biotech
 
CHGB ELISA Kit (Human) (GWB-KBBPX3)
Data Sheet
 
Product Number GWB-KBBPX3
Product Page www.genwaybio.com/chgb-elisa-kit-human-gwb-kbbpx3
Name CHGB ELISA Kit (Human) (GWB-KBBPX3)
Size 96 Wells
Sample Type serum, plasma, tissue homogenates and other biological fluids
Alias Symbols CgB, Chromogranin-B, SCG1, Secretogranin-1, Secretogranin I, SgI
Assay Info Assay Methodology: Quantitative Sandwich ELISA
NCBI Gene Id 1114
Gene Symbol CHGB
Protein Name Secretogranin-1
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Sensitivity < 0.255 ng/mL
Kit Component
ComponentAmount
Anti-CHGB Microplate96 Wells (12 x 8 Well Strips)
CHGB Lyophilized Standard2 x 40 ng
100X Biotinylated CHGB Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Kit Detection Method Colorimetric, OD 450 nm
Kit Duration ~ 3 Hours
Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)78-98%83-93%91-105%84-104%
EDTA Plasma (n=5)81-91%90-98%82-102%95-103%
Heparin Plasma (n=5)81-104%90-98%78-95%95-103%
Kit Principle Genway Biotech CHGB ELISA Kit (Human) (GWB-KBBPX3) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CHGB has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CHGB is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CHGB captured in the well.
Kit Range 0.625 - 40 ng/mL
Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)80-9482
EDTA Plasma (n=5)87-10193
Heparin Plasma (n=5)95-105101
Nucleotide Accession Num NM_001819
Protein Accession # NP_001810
Swissprot Id P05060
Datasheets/Manuals Printable datasheet for GWB-KBBPX3
Notice This exact product is available to purchase through Aviva Systems Biology.
Please see item number OKCD01710 to view product details and for ordering information.
Description of Target Secretogranin-1 is a neuroendocrine secretory granule protein, which may be the precursor for other biologically active peptides.
Species Reactivity Human
Image 1
Standard Curve
CHGB ELISA Kit (Human) (GWB-KBBPX3) standard curve.
 

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