| Product Number |
GWB-KBBPX3 |
| Product Page |
www.genwaybio.com/chgb-elisa-kit-human-gwb-kbbpx3 |
| Name |
CHGB ELISA Kit (Human) (GWB-KBBPX3) |
| Size |
96 Wells |
| Sample Type |
serum, plasma, tissue homogenates and other biological fluids |
| Alias Symbols |
CgB, Chromogranin-B, SCG1, Secretogranin-1, Secretogranin I, SgI |
| Assay Info |
Assay Methodology: Quantitative Sandwich ELISA |
| NCBI Gene Id |
1114 |
| Gene Symbol |
CHGB |
| Protein Name |
Secretogranin-1 |
| Reconstitution and Storage |
Store at 2 - 8C. Do not use the kit beyond the expiration date. |
| Sensitivity |
< 0.255 ng/mL |
| Kit Component |
| Component | Amount |
|---|
| Anti-CHGB Microplate | 96 Wells (12 x 8 Well Strips) |
| CHGB Lyophilized Standard | 2 x 40 ng |
| 100X Biotinylated CHGB Detector Antibody | 120 uL |
| Avidin/HRP Conjugate | 120 uL |
| Standard Diluent | 1 x 20 mL |
| Detector Antibody Diluent | 1 x 12 mL |
| Conjugate Diluent | 1 x 12 mL |
| 30X Wash Buffer | 1 x 20 mL |
| TMB Substrate | 1 x 9 mL |
| Stop Solution | 1 x 6 mL |
|
| Kit Detection Method |
Colorimetric, OD 450 nm |
| Kit Duration |
~ 3 Hours |
| Kit Linearity |
| Sample | 1:2 Dilution | 1:4 Dilution | 1:8 Dilution | 1:16 Dilution |
|---|
| Serum (n=5) | 78-98% | 83-93% | 91-105% | 84-104% |
| EDTA Plasma (n=5) | 81-91% | 90-98% | 82-102% | 95-103% |
| Heparin Plasma (n=5) | 81-104% | 90-98% | 78-95% | 95-103% |
|
| Kit Principle |
Genway Biotech CHGB ELISA Kit (Human) (GWB-KBBPX3) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CHGB has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CHGB is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CHGB captured in the well. |
| Kit Range |
0.625 - 40 ng/mL |
| Kit Recovery |
| Matrix | Recovery Range (%) | Average (%) |
|---|
| Serum (n=5) | 80-94 | 82 |
| EDTA Plasma (n=5) | 87-101 | 93 |
| Heparin Plasma (n=5) | 95-105 | 101 |
|
| Nucleotide Accession Num |
NM_001819 |
| Protein Accession # |
NP_001810 |
| Swissprot Id |
P05060 |
| Datasheets/Manuals |
Printable datasheet for GWB-KBBPX3 |
| Notice |
This exact product is available to purchase through Aviva Systems Biology.
Please see item number OKCD01710 to view product details and for ordering information. |
| Description of Target |
Secretogranin-1 is a neuroendocrine secretory granule protein, which may be the precursor for other biologically active peptides. |
| Species Reactivity |
Human |
| Image 1 | Standard Curve
 | CHGB ELISA Kit (Human) (GWB-KBBPX3) standard curve. |
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