||CRY1 ELISA Kit (Human) (GWB-KBBTB1)
||tissue homogenates, cell lysates and other biological fluids
||Assay Methodology: Quantitative Sandwich ELISA
|NCBI Gene Id
|Reconstitution and Storage
||Store at 2 - 8C. Do not use the kit beyond the expiration date.
||< 0.129 ng/mL
|Replacement Aviva Sku
|Anti-CRY1 Microplate||96 Wells (12 x 8 Well Strips)|
|CRY1 Lyophilized Standard||2 x 20 ng|
|100X Biotinylated CRY1 Detector Antibody||120 uL|
|Avidin/HRP Conjugate||120 uL|
|Standard Diluent||1 x 20 mL|
|Detector Antibody Diluent||1 x 12 mL|
|Conjugate Diluent||1 x 12 mL|
|30X Wash Buffer||1 x 20 mL|
|TMB Substrate||1 x 9 mL|
|Stop Solution||1 x 6 mL|
|Kit Detection Method
||Colorimetric, OD 450 nm
||~ 3 Hours
||Genway Biotech CRY1 ELISA Kit (Human) (GWB-KBBTB1) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CRY1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CRY1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CRY1 captured in the well.
||0.312 - 20 ng/mL
|Nucleotide Accession Num
|Protein Accession #
||Printable datasheet for GWB-KBBTB1
||This exact product is available to purchase through Aviva Systems Biology.
Please see item number OKCD01672 to view product details and for ordering information.
|Description of Target
||Transcriptional repressor which forms a core component of the circadian clock.
|Replacement Aviva Sku Url
|Standard Curve||CRY1 ELISA Kit (Human) (GWB-KBBTB1) standard curve.|