||LDB1 ELISA Kit (Human) (GWB-KBBTF7)
||Serum, plasma, tissue homogenates
||Carboxyl-terminal LIM domain-binding protein 2, CLIM2, CLIM-2, hLdb1, LDB-1, LIM domain-binding factor CLIM2, LIM domain-binding protein 1, NLI, Nuclear LIM interactor
||Assay Methodology: Quantitative Sandwich ELISA
|NCBI Gene Id
||LIM domain-binding protein 1
|Reconstitution and Storage
||Store at 2 - 8C. Do not use the kit beyond the expiration date.
||< 0.061 ng/mL
|Anti-LDB1 Microplate||96 Wells (12 x 8 Well Strips)|
|LDB1 Lyophilized Standard||2 x 10 ng|
|Standard Diluent||1 x 20 mL|
|100X LDB1 Biotinylated Detector Antibody||1 x 120 µL|
|100X Avidin-HRP Detection Conjugate||1 x 120 µL|
|Detector Antibody Diluent||1 x 12 mL|
|Detection Conjugate Diluent||1 x 12 mL|
|10X Sample Diluent||1 x 30 mL|
|30X Wash Buffer||1 x 20 mL|
|TMB Substrate||1 x 9 mL|
|Stop Solution||1 x 6 mL|
|Kit Detection Method
||Absorbance, OD 450 nm
||~ 3.5 Hours
||Genway Biotech LDB1 ELISA Kit (Human) (GWB-KBBTF7) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for LDB1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for LDB1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample LDB1 captured in the well.
|Nucleotide Accession Num
|Protein Accession #
||Printable datasheet for GWB-KBBTF7
||This exact product is now provided by Aviva Systems Biology.
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|Description of Target
||Binds to the LIM domain of a wide variety of LIM domain-containing transcription factors. May regulate the transcriptional activity of LIM-containing proteins by determining specific partner interactions. Plays a role in the development of interneurons an
|Standard Curve||LDB1 ELISA Kit (Human) (GWB-KBBTF7) Standard Curve|