Product Datasheet: GWB-KBBTF7 - LDB1 ELISA Kit (Human) (GWB-KBBTF7) - Genway Biotech
 
LDB1 ELISA Kit (Human) (GWB-KBBTF7)
Data Sheet
 
Product Number GWB-KBBTF7
Product Page www.genwaybio.com/ldb1-elisa-kit-human-gwb-kbbtf7
Name LDB1 ELISA Kit (Human) (GWB-KBBTF7)
Size 96 Wells
Sample Type Serum, plasma, tissue homogenates
Alias Symbols Carboxyl-terminal LIM domain-binding protein 2, CLIM2, CLIM-2, hLdb1, LDB-1, LIM domain-binding factor CLIM2, LIM domain-binding protein 1, NLI, Nuclear LIM interactor
Assay Info Assay Methodology: Quantitative Sandwich ELISA
NCBI Gene Id 8861
Gene Symbol LDB1
Protein Name LIM domain-binding protein 1
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Sensitivity < 0.061 ng/mL
Kit Component
ComponentAmount
Anti-LDB1 Microplate96 Wells (12 x 8 Well Strips)
LDB1 Lyophilized Standard2 x 10 ng
Standard Diluent1 x 20 mL
100X LDB1 Biotinylated Detector Antibody1 x 120 µL
100X Avidin-HRP Detection Conjugate1 x 120 µL
Detector Antibody Diluent1 x 12 mL
Detection Conjugate Diluent1 x 12 mL
10X Sample Diluent1 x 30 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Kit Detection Method Absorbance, OD 450 nm
Kit Duration ~ 3.5 Hours
Kit Principle Genway Biotech LDB1 ELISA Kit (Human) (GWB-KBBTF7) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for LDB1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for LDB1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample LDB1 captured in the well.
Kit Range 0.156-10 ng/mL
Nucleotide Accession Num NM_001113407.2
Protein Accession # NP_001106878.1
Swissprot Id Q86U70
Datasheets/Manuals Printable datasheet for GWB-KBBTF7
Notice This exact product is now provided by Aviva Systems Biology.
Please visit OKDD00373 to review further and for ordering information.
Description of Target Binds to the LIM domain of a wide variety of LIM domain-containing transcription factors. May regulate the transcriptional activity of LIM-containing proteins by determining specific partner interactions. Plays a role in the development of interneurons an
Species Reactivity Human
Image 1
Standard Curve
LDB1 ELISA Kit (Human) (GWB-KBBTF7) Standard Curve
 

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