| Product Number |
GWB-KBBTL8 |
| Product Page |
www.genwaybio.com/igg3-elisa-kit-human-gwb-kbbtl8 |
| Name |
IgG3 ELISA Kit (Human) (GWB-KBBTL8) |
| Size |
96 Wells |
| Sample Type |
Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernatant |
| Alias Symbols |
DKFZp686H11213, FLJ39988, FLJ40036, FLJ40253, FLJ40587, FLJ40789, FLJ40834, HDC, Heavy chain disease protein, IgG3, Ig gamma-3 chain C region, MGC45809 |
| Assay Info |
Assay Methodology: Quantitative Sandwich ELISA |
| NCBI Gene Id |
3502 |
| Gene Symbol |
IgG3 |
| Protein Name |
IgG3 |
| Reconstitution and Storage |
Store at 2 - 8C. Do not use the kit beyond the expiration date. |
| Sensitivity |
< 0.055 ng/mL |
| Kit Component |
| Component | Amount |
| Anti-IgG3 Microplate | 96 Wells (12 x 8 Well Strips) |
| IgG3 Lyophlized Standard | 2 x 10 ng |
| Standard Diluent | 1 x 20 mL |
| 100X IgG3 Biotinylated Detector Antibody | 1 x 120 µL |
| 100X Avidin-HRP Detection Conjugate | 1 x 120 µL |
| Detector Antibody Diluent | 1 x 12 mL |
| Detection Conjugate Diluent | 1 x 12 mL |
| 10X Sample Diluent | 1 x 30 mL |
| 30X Wash Buffer | 1 x 20 mL |
| TMB Substrate | 1 x 9 mL |
| Stop Solution | 1 x 6 mL | |
| Kit Detection Method |
Absorbance, OD 450 nm |
| Kit Duration |
~ 3.5 Hours |
| Kit Principle |
Genway Biotech IgG3 ELISA Kit (Human) (GWB-KBBTL8) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for IgG3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for IgG3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample IgG3 captured in the well. |
| Kit Range |
0.156-10 ng/mL |
| Swissprot Id |
P01860 |
| Datasheets/Manuals |
Printable datasheet for GWB-KBBTL8 |
| Notice |
This exact product is now provided by Aviva Systems Biology.
Please visit OKDD00330 to review further and for ordering information. |
| Species Reactivity |
Human |
| Image 1 | Standard Curve
 | IgG3 ELISA Kit (Human) (GWB-KBBTL8) Standard Curve |
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