Product Datasheet: GWB-KBBUU3 - Zp2 ELISA Kit (Rat) (GWB-KBBUU3) - Genway Biotech
 
Zp2 ELISA Kit (Rat) (GWB-KBBUU3)
Data Sheet
 
Product Number GWB-KBBUU3
Product Page www.genwaybio.com/zp2-elisa-kit-rat-gwb-kbbuu3
Name Zp2 ELISA Kit (Rat) (GWB-KBBUU3)
Sample Type tissue homogenates, cell lysates and other biological fluids.
Alias Symbols Zona pellucida glycoprotein 2, Zona pellucida protein A, Zona pellucida sperm-binding protein 2, Zp-2, Zpa
Assay Info Assay Methodology: Quantitative Sandwich ELISA
NCBI Gene Id 81828
Gene Symbol ZP2
Protein Name zona pellucida sperm-binding protein 2
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Sensitivity < 0.125 ng/mL
Kit Component
ComponentAmount
Anti-NPM1 Microplate96 Wells (12 x 8 Well Strips)
NPM1 Lyophilized Standard2 x 20 ng
100X Biotinylated NPM1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Kit Detection Method Colorimetric, OD 450 nm
Kit Duration ~ 3 Hours
Kit Principle Genway Biotech Zp2 ELISA Kit (Rat) (GWB-KBBUU3) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Zp2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Zp2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Zp2 captured in the well.
Kit Range 0.312 - 20 ng/mL
Nucleotide Accession Num NM_031150
Protein Accession # NP_112412
Swissprot Id O54767
Datasheets/Manuals Printable datasheet for GWB-KBBUU3
Notice This exact product is now provided by Aviva Systems Biology.
Please visit OKCD00875 to review further and for ordering information.
Description of Target The mammalian zona pellucida, which mediates species-specific sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy, is composed of three to four glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP2 may act as a secondary sper
Species Reactivity Rat
Image 1
Standard Curve
Zp2 ELISA Kit (Rat) (GWB-KBBUU3) standard curve.
 

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