Product Datasheet: GWB-KBBXA2 - CALML3 ELISA Kit (Human) (GWB-KBBXA2) - Genway Biotech
Data Sheet
Product Number GWB-KBBXA2
Product Page
Name CALML3 ELISA Kit (Human) (GWB-KBBXA2)
Size 96 Wells
Sample Type Serum, Plasma, Tissue homogenates
Alias Symbols Calmodulin-like protein 3, Calmodulin-related protein NB-1, CaM-like protein, CLP
Assay Info Assay Methodology: Quantitative Sandwich ELISA
NCBI Gene Id 810
Gene Symbol CALML3
Protein Name Calmodulin-like protein 3
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Sensitivity < 0.054 ng/mL
Kit Component
Anti-CALML3 Microplate96 Wells (12 x 8 Well Strips)
CALML3 Lyophlized Standard2 x 10 ng
Standard Diluent1 x 20 mL
100X CALML3 Biotinylated Detector Antibody1 x 120 µL
100X Avidin-HRP Detection Conjugate1 x 120 µL
Detector Antibody Diluent1 x 12 mL
Detection Conjugate Diluent1 x 12 mL
10X Sample Diluent1 x 30 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Kit Detection Method Absorbance, OD 450 nm
Kit Duration ~ 3.5 Hours
Kit Principle Genway Biotech CALML3 ELISA Kit (Human) (GWB-KBBXA2) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CALML3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CALML3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CALML3 captured in the well.
Kit Range 0.156-10 ng/mL
Nucleotide Accession Num NM_005185.3
Protein Accession # NP_005176.1
Swissprot Id P27482
Datasheets/Manuals Printable datasheet for GWB-KBBXA2
Notice This exact product is now provided by Aviva Systems Biology.
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Species Reactivity Human
Image 1
Standard Curve
CALML3 ELISA Kit (Human) (GWB-KBBXA2) Standard Curve

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