Product Datasheet: GWB-SKR076 - Mouse IFN-gamma ELISA Kit (GWB-SKR076) - Genway Biotech
 
Mouse IFN-gamma ELISA Kit (GWB-SKR076)
Data Sheet
 
Product Number GWB-SKR076
Product Page www.genwaybio.com/mouse-ifn-gamma-elisa-kit-80874
Name Mouse IFN-gamma ELISA Kit (GWB-SKR076)
Reactivity Mouse
Size 96 Wells
Related Product Names (Synonyms) Interferon gamma
GI Number 15978
Stability 6 months
Format ELISA Kit. Refer to manual for specific component formats
Storage 4C/6 Months
Kit Size 12 x 8-Well Microstrips
Standard Range 23-1500 pg/ml
Sample Type Cell lysates, sera and plasma
Specimen/Volumes Refer to Manual
Intended Use Research Use Only
Alias Symbols Ifg, IFN-g, IFN-gamma, Interferon gamma
Assay Info Quantitative Colorimentric Sandwich ELISA
Gene Family IFN
Official Gene Full Name interferon gamma
NCBI Gene Id 15978
Gene Symbol IFNG
Product Format 1 x 96-Well Plate or 12 x 8-Well Strips
Sensitivity 23 pg/mL
Description Short Description: This product is a Sandwich ELISA with a detection method of Colorimetric 450 nm. The Murine IFN-gamma ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Murine IFN-gamma proteins within the range of 23-1500 pg/ml. The Murine IFN-gamma ELISA is capable of recognizing both recombinant and naturally produced Murine IFN-gamma proteins. The antigens listed below were tested at 50 ng/ml and exhibited 100% cross reactivity. • Rat IFN-gamma The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross reactivity or interference. • Human IFN-alpha, IFN-beta, IFN-gamma, IFN-?1, IFN-?2, IL-10, IL-18 • Murine IFN-?2, IL-10, IL-12, IL-18 • Rat IL-10
Kit Component
ComponentsAmount
Capture Antibody Coated Microplate96 Wells (12 x 8)
Ready-to-Use Streptavidin-HRP12 mL
Ready-to-Use Substrate12 mL
Stop Solution12 mL
Wash Buffer (15x)50 mL
Protein Standard Diluent12 mL
Sample Diluent12 mL
Detection Antibody Diluent12 mL
Biotin-Labeled Detection AntibodyLyophilized
Protein StandardLyophilized (100 ng)
Kit Detection Method Colorimetric 450 nm
Kit Principle The Aviva Murine IFN-? ELISA Kit contains the components necessary for quantitative determination of natural or recombinant mIFN-? concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on the Murine IFN-? cytokine while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a colorimetric reaction to ensue upon substrate addition. When the substrate TMB (3, 3’, 5, 5’-Tetramethylbenzidine) is added, the reaction catalyzed by peroxidase yields a blue color that is representative of the antigen concentration. Upon sufficient color development, the reaction can be terminated through addition of Stop Solution (2 N Sulfuric Acid) where the color of the solution will turn yellow. The absorbance of each well can then be read by a spectrophotometer, allowing for generation of a standard curve and subsequent determination of protein concentration.
Kit Range 23-1500 pg/mL
Lead Time Domestic: within 1 week delivery | International: 1 week
Nucleotide Accession Num NM_008337.4
Protein Accession # NP_032363.1
Protein Name Interferon gamma
Reconstitution and Storage 4C/6 Months
Specificity The Murine IFN-? ELISA is capable of recognizing both recombinant and naturally produced Murine IFN-? proteins. The antigens listed below were tested at 50 ng/ml and exhibited 100% cross reactivity.
• Rat: IFN-?
The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross reactivity or interference.
• Human: IFN-?, IFN-?, IFN-?, IFN-?1, IFN-?2, IL-10, IL-18
• Murine: IFN-?2, IL-10, IL-12, IL-18
• Rat: IL-10
Swissprot Id P01580
Datasheets/Manuals Printable datasheet for GWB-SKR076
Notice This exact product is available to purchase through Aviva Systems Biology.
Please see item number OKAG00076 to view product details and for ordering information.
Description of Target This gene encodes a soluble cytokine that is a member of the type II interferon class. The encoded protein is secreted by cells of both the innate and adaptive immune systems. The active protein is a homodimer that binds to the interferon gamma receptor w
Species Reactivity Mouse
Application ELISA
Image 1
Standard Curve
IFN-? ELISA Kit (Mouse) (OKAG00076) Standard Curve
 

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