Product Datasheet: GWB-SKR195 - Human Angiopoietin-2 ELISA Kit (GWB-SKR195) - Genway Biotech
 
Human Angiopoietin-2 ELISA Kit (GWB-SKR195)
Data Sheet
 
Product Number GWB-SKR195
Product Page www.genwaybio.com/human-angiopoietin-2-elisa-kit
Name Human Angiopoietin-2 ELISA Kit (GWB-SKR195)
Reactivity Human
Size 96 Wells
Related Product Names (Synonyms) ANG-2
GI Number 285
Stability 6 months
Format ELISA Kit. Refer to manual for specific component formats
Storage 4C/6 Months
Kit Size 12 x 8-Well Microstrips
Standard Range 94-6000 pg/ml
Sample Type Cell lysates, sera and plasma
Specimen/Volumes Refer to Manual
Intended Use Research Use Only
Alias Symbols AGPT2, Ang2, ANG2, ANG-2, Angiopoietin-2
Assay Info Quantitative Colorimentric Sandwich ELISA
Gene Family FIBC
Official Gene Full Name angiopoietin 2
NCBI Gene Id 285
Gene Symbol ANGPT2
Product Format 1 x 96-Well Plate or 12 x 8-Well Strips
Sensitivity 94 pg/mL
Description Short Description: This product is a Sandwich ELISA with a detection method of Colorimetric 450 nm. The Human Angiopoietin-2 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human Angiopoietin-2 proteins within the range of 94-6000 pg/ml. The Human Angiopoietin-2 ELISA is capable of recognizing both recombinant and naturally produced Human Angiopoietin-2 proteins. The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross-reactivity or interference. • Human Ang-1, FGF basic (146 aa), FGF basic (157 aa), Tie-1, Tie-2, TNF-alpha, VEGF121, VEGF165 • Murine Ang-3, Tie-2
Kit Component
ComponentsAmount
Capture Antibody Coated Microplate96 Wells (12 x 8)
Ready-to-Use Streptavidin-HRP12 mL
Ready-to-Use Substrate12 mL
Stop Solution12 mL
Wash Buffer (15x)50 mL
Protein Standard Diluent12 mL
Sample Diluent12 mL
Detection Antibody Diluent12 mL
Biotin-Labeled Detection AntibodyLyophilized
Protein StandardLyophilized (32 ng)
Kit Detection Method Colorimetric 450 nm
Kit Principle The Aviva Human Angiopoietin-2 ELISA Kit contains the components necessary for quantitative determination of natural or recombinant hAngiopoietin-2 concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on the Human Angiopoietin-2 cytokine while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a colorimetric reaction to ensue upon substrate addition. When the substrate TMB (3, 3’, 5, 5’-Tetramethylbenzidine) is added, the reaction catalyzed by peroxidase yields a blue color that is representative of the antigen concentration. Upon sufficient color development, the reaction can be terminated through addition of Stop Solution (2 N Sulfuric Acid) where the color of the solution will turn yellow. The absorbance of each well can then be read by a spectrophotometer, allowing for generation of a standard curve and subsequent determination of protein concentration.
Kit Range 94-6,000 pg/mL
Nucleotide Accession Num NM_001118887.1
Protein Accession # NP_001112359.1
Protein Name Angiopoietin-2
Reconstitution and Storage 4C/6 Months
Specificity The Human Angiopoietin-2 ELISA is capable of recognizing both recombinant and naturally produced Human Angiopoietin-2 proteins. The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross-reactivity or interference.
• Human: Ang-1, FGF basic (146 aa), FGF basic (157 aa), Tie-1, Tie-2, TNF-?, VEGF121, VEGF165
• Murine: Ang-3, Tie-2
Swissprot Id O15123
Datasheets/Manuals Printable datasheet for GWB-SKR195
Notice This exact product is now provided by Aviva Systems Biology.
Please visit OKAG00195 to review further and for ordering information.
Description of Target The protein encoded by this gene is an antagonist of angiopoietin 1 (ANGPT1) and endothelial TEK tyrosine kinase (TIE-2, TEK). The encoded protein disrupts the vascular remodeling ability of ANGPT1 and may induce endothelial cell apoptosis. Three transcri
Species Reactivity Human
Application ELISA
Image 1
Standard Curve
Angiopoietin-2 ELISA Kit (Human) (OKAG00195) Standard Curve
 

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