Product Datasheet: GWB-ZZD043 - Human ICAM-1 ELISA Kit (GWB-ZZD043) - Genway Biotech
 
Human ICAM-1 ELISA Kit (GWB-ZZD043)
Data Sheet
 
Product Number GWB-ZZD043
Product Page www.genwaybio.com/human-icam-1-elisa-kit
Name Human ICAM-1 ELISA Kit (GWB-ZZD043)
Reactivity Reactive to Human. There is no detectable cross-reactivity with other relevant proteins.
Size 96 Tests
Concentration Lot Specific
Related Product Names (Synonyms) BB2| CD 54| CD54| CD54 antigen| Cell surface glycoprotein P3.58| Human rhinovirus receptor| ICAM 1| Intercellular adhesion molecule 1| Major group rhinovirus receptor| MALA2| MGC6195| MyD10| P3.58| Surface antigen of activated B cells
GI Number 3383
Stability Four months at 4C and eight months at -20C.
Storage Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
Kit Size 1x96 well plate
Standard Range 156pg/ml-10,000pg/ml. Test has a sensitivity of < 10 pg/ml
Sample Type cell culture supernates, serum and plasma (heparin, EDTA)
Specimen/Volumes Refer to Manual
Intended Use Research Use Only
Alias Symbols Intercellular adhesion molecule 1, Major group rhinovirus receptor, CD54
Official Gene Full Name intercellular adhesion molecule 1
NCBI Gene Id 3383
Gene Symbol ICAM1
Sensitivity < 10 pg/ml
Description Short Description: Intercellular adhesion molecule-1(ICAM-1) is an integral membrane protein, a member of the immunoglobulin superfamily, and a ligand for lymphocyte function-associated(LFA) antigens, a beta 2 leukocyte integrin. The normal function of human intercellular adhesion molecule-1(ICAM-1) is to provide adhesion between endothelial cells and leukocytes after injury or stress. ICAM-1 binds to leukocyte function-associated antigen(LFA-1) or macrophage-1 antigen(Mac-1). ICAM-1 is found on leukocytes, fibroblasts, epithelial cells and endothelial cells and its expression is regulated by inflammatory cytokines. ICAM-1 has a tissue distribution similar to that of the major histocompatibility complex class II antigens and is likely to play a role in inflammatory responses.1
Additional Information Range: 156pg/ml-10,000pg/ml
Additional Information Principle: Aviva’s human ICAM-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for ICAM-1 has been precoated onto 96-well plates. Standards (NSO,Q28-E480) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ICAM-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the
human ICAM-1 amount of sample captured in plate.
Additional Information Notes: 1. To inspect the validity of experiment operation and the appropriateness of sample dilution proportion, pilot
experiment using standards and a small number of samples is recommended.
2. The TMB Color Developing agent is colorless and transparent before using, contact us freely if it is not the case.
3. Before using the Kit, spin tubes and bring down all components to the bottom of tubes.
4. Duplicate well assay is recommended for both standard and sample testing.
5. Don’t let 96-well plate dry, for dry plate will inactivate active components on plate.
6. Don’t reuse tips and tubes to avoid cross contamination.
7. To avoid to use the reagents from different batches together.
8. In order to avoid marginal effect of plate incubation due to temperature difference ( reaction may be stronger in the
marginal wells), it is suggested that the diluted ABC and TMB solution will be pre-warmed in 37C for 30 min before
using.
Background: Intercellular adhesion molecule-1 (ICAM-1) is an integral membrane protein, a member of the immunoglobulin superfamily, and a ligand for lymphocyte function-associated (LFA) antigens, a beta 2 leukocyte integrin.1 The normal function of human intercellular adhesion molecule-1 (ICAM-1) is to provide adhesion between endothelial cells and leukocytes after injury or stress. ICAM-1 binds to leukocyte function-associated antigen (LFA-1) or macrophage-1 antigen (Mac-1).2 ICAM-1 is found on leukocytes, fibroblasts, epithelial cells and endothelial cells and its expression is regulated by inflammatory cytokines.3 ICAM-1 has a tissue distribution similar to that of the major histocompatibility complex class II antigens and is likely to play a role in inflammatory responses.1 The standard product used in this kit is produced by recombinant gene expression, consisting of 455 amino acids with the molecular mass of 50kDa.
Target Reference 1. Ballantyne, C. M.; Kozak, C. A.; O'Brien, W. E.; Beaudet, A. L. Assignment of the gene for intercellular adhesion
molecule-1 (Icam-1) to proximal mouse chromosome 9. Genomics 9: 547-550, 1991.
2. Bella, J.; Kolatkar, P. R.; Marlor, C. W.; Greve, J. M.; Rossmann, M. G. The structure of the two amino-terminal
domains of human ICAM-1 suggests how it functions as a rhinovirus receptor and as an LFA-1 integrin ligand. Proc.
Nat. Acad. Sci. 95: 4140-4145, 1998.
3. Simmons, D.; Makgoba, M. W.; Seed, B. ICAM, an adhesion ligand of LFA-1, is homologous to the neural cell
adhesion molecule NCAM. Nature 331: 624-627, 1988.
Kit Component
ComponentAmount
Lyophilized recombinant mouse Fractalkine standard10 ng/tube x 2
Anti-mouse Fractalkine Antibody Well Plate96 Wells
Sample diluent buffer30 mL
Biotinylated anti- mouse Fractalkine antibody130 uL, dilution 1:100
Antibody diluent buffer12 mL
Avidin-Biotin-Peroxidase Complex (ABC)130 uL, dilution 1:100
ABC diluent buffer12 mL
TMB color developing agent10 mL
TMB stop solution10 mL
Kit Detection Method Colorimetric, OD 450 nm
Kit Duration ~ 3 Hours
Kit Principle Genway Biotech ICAM1 ELISA Kit (Human) (GWB-ZZD043) is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody specific for ICAM1 has been pre-coated onto 96-wellplate (12 x 8 Well Strips). Standards (NSO, Q28-E480) and test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ICAM1 is added, incubated and followed by washing. Avidin-Biotin-Peroxidase Complex is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is visualized through the addition of TMB substrate which is catalyzed by HRP to produce a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Human ICAM1 captured in well.
Kit Range 156 pg/ml-10,000 pg/ml
Nucleotide Accession Num NM_000201.2
Protein Accession # NP_000192.2
Protein Name Intercellular adhesion molecule 1
Reconstitution and Storage Store at 4C for frequent use, at -20C for infrequent use.
Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Specificity There is no detectable cross-reactivity with other relevant proteins.
Swissprot Id P05362
Datasheets/Manuals Printable datasheet for GWB-ZZD043
Notice This exact product is now provided by Aviva Systems Biology.
Please visit OKBB00158 to review further and for ordering information.
Immunogen NSO, Q28-E480
Description of Target Intercellular adhesion molecule-1 (ICAM-1) is an integral membrane protein, a member of the immunoglobulin superfamily, and a ligand for lymphocyte function-associated (LFA) antigens, a beta 2 leukocyte integrin.1 The normal function of human intercellula
Species Reactivity Human
Predicted Homology Based on Immunogen Sequence No detectable cross-reactivity with any other cytokine.
Application ELISA
Image 1
ICAM1 ELISA standard curve
Human ICAM1 ELISA Kit standard curve
 

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