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96 Wells
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Additional Information

Name H2AFV ELISA Kit (Human) (GWB-KBBIV2)
Kit Range 0.156-10 ng/mL
Sensitivity < 0.057 ng/mL
Sample Type Serum, Plasma, Tissue homogenates, Cell lysates
Kit Detection Absorbance, OD 450 nm
Gene Symbol H2AFV
Gene id 94239
Alias Symbols FLJ26479, H2A.F/Z, H2AV, Histone H2A.V, MGC10170, MGC10831, MGC1947
Swissprot ID Q71UI9
Protein Name histone H2A.V isoform 1
Description of Target Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core hi
Kit Principle Genway Biotech H2AFV ELISA Kit (Human) (GWB-KBBIV2) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for H2AFV has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for H2AFV is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample H2AFV captured in the well.
Kit Component
Anti-H2AFV Microplate96 Wells (12 x 8 Well Strips)
H2AFV Lyophlized Standard2 x 10 ng
Standard Diluent1 x 20 mL
100X H2AFV Biotinylated Detector Antibody1 x 120 µL
100X Avidin-HRP Detection Conjugate1 x 120 µL
Detector Antibody Diluent1 x 12 mL
Detection Conjugate Diluent1 x 12 mL
10X Sample Diluent1 x 30 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_036544.1
Nucleotide Accession Num NM_012412.4
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3.5 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBIV2
Species Reactivity Human