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96 Wells
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Additional Information

Name H2AFV ELISA Kit (Human) (GWB-KBBLG2)
Kit Range 1.56 - 100 ng/mL
Sensitivity < 0.59 ng/mL
Sample Type tissue homogenates, cell lysates and other biological fluids
Kit Detection Colorimetric, OD 450 nm
Gene Symbol H2AFV
Gene id 94239
Alias Symbols FLJ26479, H2A.F/Z, H2AV, Histone H2A.V, MGC10170, MGC10831, MGC1947
Swissprot ID Q71UI9
Protein Name histone H2A.V
Description of Target Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role i
Kit Principle Genway Biotech H2AFV ELISA Kit (Human) (GWB-KBBLG2) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for H2AFV has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for H2AFV is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample H2AFV captured in the well.
Kit Component
Anti-PIK3C3 Microplate96 Wells (12 x 8 Well Strips)
PIK3C3 Lyophilized Standard2 x 100 ng
100X Biotinylated PIK3C3 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_036544
Nucleotide Accession Num NM_012412
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBLG2
Species Reactivity Human