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96 Wells
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Additional Information

Name H2AFX ELISA Kit (Human) (GWB-KBBGN3)
Kit Range 0.156 - 10 ng/mL
Sensitivity < 0.059 ng/mL
Sample Type tissue homogenates, cell lysates cell culture supernatants and other biological fluids
Kit Detection Colorimetric, OD 450 nm
Gene Symbol H2AFX
Gene id 3014
Alias Symbols H2a/x, H2A/X, H2A.X, H2AX, Histone H2A.x
Swissprot ID P16104
Protein Name Histone H2AX
Description of Target Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role i
Kit Principle Genway Biotech H2AFX ELISA Kit (Human) (GWB-KBBGN3) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for H2AFX has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for H2AFX is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample H2AFX captured in the well.
Kit Component
Anti-H2AFX Microplate96 Wells (12 x 8 Well Strips)
H2AFX Lyophilized Standard2 x 10 ng
100X Biotinylated H2AFX Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_002096
Nucleotide Accession Num NM_002105
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBGN3
Species Reactivity Human